Tuneable 3D printed bioreactors for transaminations under continuous-flow
Tuneable 3D printed bioreactors for transaminations under continuous-flow
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DOI：
10.1039/c7gc02421e
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发表时间：
2017-11-21
期刊：
影响因子：
9.8
通讯作者：
Sans, Victor
中科院分区：
文献类型：
--
作者：
Peris, Edgar;Okafor, Obinna;Sans, Victor
提出了一种在3D打印连续流动装置上有效地将酶固定化的方法。这些化学改性装置的应用使得能够快速筛选固定化机制和反应条件，将优化条件简单地转移到定制的印刷微流体反应器中，并开发连续流生物催化过程。该生物反应器在1-甲基苄胺的动力学拆分中表现出良好的活性（8-20.5 μ mol h（-1）mg（enz）（-1））和非常好的稳定性（约100 μ mol h（-1））。100小时下流动）。
A method to efficiently immobilize enzymes on 3D printed continuous-flow devices is presented. Application of these chemically modified devices enables rapid screening of immobilization mechanisms and reaction conditions, simple transfer of optimised conditions into tailored printed microfluidic reactors and development of continuous-flow biocatalytic processes. The bioreactors showed good activity (8-20.5 mu mol h(-1) mg(enz)(-1)) in the kinetic resolution of 1-methylbenzylamine, and very good stability (ca. 100 h under flow).