BRAIN-DEVELOPMENT - H-1 MAGNETIC-RESONANCE SPECTROSCOPY OF RAT-BRAIN EXTRACTS COMPARED WITH CHROMATOGRAPHIC METHODS
BRAIN-DEVELOPMENT - H-1 MAGNETIC-RESONANCE SPECTROSCOPY OF RAT-BRAIN EXTRACTS COMPARED WITH CHROMATOGRAPHIC METHODS
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DOI：
10.1007/bf00965747
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发表时间：
1990-10-01
影响因子：
4.4
通讯作者：
HERSCHKOWITZ, N
中科院分区：
文献类型：
--
作者：
BURRI, R;BIGLER, P;HERSCHKOWITZ, N
我们将大鼠脑提取物（大鼠：2-56 天）的体外 H-1 磁共振波谱 (MRS) 测量结果与色谱测量结果进行比较，并进一步与体内 MRS 的结果进行比较。 通过体外 MRS 可以可靠地测量脑提取物中的以下物质：N-乙酰天冬氨酸 (NAA)、总肌酸 (Cr)、磷酸乙醇胺 (PE)、牛磺酸 (Tau)、谷氨酸 (Glu)、谷氨酰胺 (Gln)、γ-氨基丁酸 (GABA) 和丙氨酸 (Ala)。 显示了与 Cr 和 NAA 色谱数据相比的两种不同 MRS 数据评估方法。 在大鼠发育过程中，从第2天到第56天，PE、Tau和Ala的脑浓度下降，NAA、Cr、Glu和Gln的浓度增加，而GABA没有变化。 无论是通过体外 MRS 还是通过色谱方法测量，这些物质的发育模式都是相同的。 两种方法对 NAA、Cr、Tau、GABA 和 PE 的定量结果相同，而体外 MRS 测定的 Glu、Gln 和 Ala 浓度明显低于化学测定的浓度。 通过体内 H-1 MRS 测定 7 至 35 日龄大鼠的 NAA/Cr 比值。 这些结果与色谱和体外数据相关。 在体内和体外 MR 技术中使用适当的方法，获得的数据与色谱结果很好地比较。
We compared in vitro H-1 magnetic resonance spectroscopy (MRS) measurements of rat brain extracts (rats: 2-56 days old) with chromatographic measurements and in a further step also with results of in vivo MRS. The following substances can be reliably measured in brain extracts by in vitro MRS: N-acetylaspartate (NAA), total creatine (Cr), phosphorylethanoloamine (PE), taurine (Tau), glutamate (Glu), glutamine (Gln), gamma-aminobutyrate (GABA) and alanine (Ala). Two different methods of MRS data evaluation compared with chromatographic data on Cr and NAA are shown. During development of the rat from day 2-56 brain concentrations of PE, Tau and Ala decrease, those of NAA, Cr, Glu and Gln increase, while GABA does not change. The developmental patterns of these substances are the same, whether measured by in vitro MRS or by chromatogrphic methods. Qualification of NAA, Cr, Tau, GABA and PE leads to the same results with both methods, while Glu, Gln and Ala concentrations determined by in vitro MRS are apparently lower than those measured chemically. The NAA/Cr ratios of 7 to 35-day-old rats were determined by in vivo H-1 MRS. These results correlate with chromatographic and in vitro data. Using appropriate methods in the in vivo and in vitro MR-technique, the obtained data compare well with the chromatographic results.