Integrated bioinformatics analysis reveals role of the LINC01093/miR-96-5p/ZFAND5/NF-κB signaling axis in hepatocellular carcinoma
Integrated bioinformatics analysis reveals role of the LINC01093/miR-96-5p/ZFAND5/NF-κB signaling axis in hepatocellular carcinoma
复制标题
综合生物信息学分析揭示了 LINC01093/miR-96-5p/ZFAND5/NF-kappa B 信号轴在肝细胞癌中的作用
DOI：
10.3892/etm.2019.8046
复制
发表时间：
2019-11-01
影响因子：
2.7
通讯作者：
Zhang, Jubo
中科院分区：
文献类型：
--
作者：
Zheng, Yahui;Yu, Kangkang;Zhang, Jubo
肝细胞癌（HCC）是世界范围内重要的健康负担，其发病机制尚未完全阐明。长链非编码（lnc） rna调控基因表达的手段之一是通过与微（mi） rna相互作用并作为竞争的内源性（ce） rna。lncrna在多种疾病中发挥着重要作用。本研究的目的是研究lncrna在HCC中的潜在作用。从Gene expression Omnibus数据库中获得21对HCC组织和邻近非肿瘤组织的RNA表达谱。差异表达的rna使用r中的DESeq软件包进行分析，选择的rna使用基因表达谱交互分析和/或Kaplan-Meier绘图仪进行表达验证和生存分析。使用lncBase或TargetScan预测mirna的靶基因。使用Database for Annotation， Visualization和Integrated Discovery进行功能分析，并使用Cytoscape确定调控网络。长基因间非蛋白编码RNA 1093 （LINC01093）被鉴定为HCC组织中最显著下调的lncrna之一。LINC01093表达下调与预后不良相关。建立包含LINC01093、miR-96-5p和锌指AN1-type containing 5 （ZFAND5）的ceRNA网络。根据功能分析，NF-kappa B信号参与了HCC的调控网络。本研究发现LINC01093/miR-96-5p/ZFAND5/NF-kappa B信号轴可能在HCC的发病过程中发挥重要作用，对该信号轴的进一步研究可能为HCC的发生发展提供新的认识。
Hepatocellular carcinoma (HCC) is a significant health burden worldwide and its pathogenesis remains to be fully elucidated. One of the means by which long non-coding (lnc)RNAs regulate gene expression is by interacting with micro (mi)RNAs and acting as competing endogenous (ce)RNAs. lncRNAs have important roles in various diseases. The aim of the present study was to examine the potential roles of lncRNAs in HCC. The RNA expression profiles of 21 paired tissues of HCC and adjacent non-tumor tissues were obtained from the Gene Expression Omnibus database. The differentially expressed RNAs were analyzed using the DESeq package in R. Expression validation and survival analysis of selected RNAs were performed using Gene Expression Profile Interactive Analysis and/or Kaplan-Meier Plotter. The target genes of the miRNAs were predicted using lncBase or TargetScan. Functional analyses were performed using the Database for Annotation, Visualization and Integrated Discovery, and regulatory networks were determined using Cytoscape. Long intergenic non-protein coding RNA 1093 (LINC01093) was identified as one of the most significantly downregulated lncRNAs in HCC tissues. Downregulated expression of LINC01093 was associated with poor prognosis. A ceRNA network involving LINC01093, miR-96-5p and zinc finger AN1-type containing 5 (ZFAND5) was established. According to functional analyses, NF-kappa B signaling was implicated in the regulatory network for HCC. The present study revealed that a LINC01093/miR-96-5p/ZFAND5/NF-kappa B signaling axis may have an important role in the pathogenesis of HCC, and further investigation of this axis may provide novel insight into the development and progression of HCC.