Observations on the type of infection by epstein‐barr virus in peripheral lymphoid cells of patients with infectious mononucleosis
Observations on the type of infection by epstein‐barr virus in peripheral lymphoid cells of patients with infectious mononucleosis
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传染性单核细胞增多症患者外周淋巴细胞EB病毒感染类型观察
DOI：
10.1002/ijc.2910140603
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发表时间：
1974
影响因子：
6.4
通讯作者：
M. Epstein
中科院分区：
文献类型：
--
作者：
A. Rickinson;Jarvis Je;D. Crawford;M. Epstein
在传染性单核细胞增多症(IM)患者外周血白细胞的培养以及IM与异性胎儿脐血白细胞的共培养中，进行了向连续细胞系的转化研究。共培养的转化细胞是混合来源的，通常以胎儿细胞为主。中和EB病毒抗血清可显著降低或消除IM白细胞培养的转化率。这种影响不是由于细胞毒性，而是遵循已知依赖于传染性EB病毒细胞间转移的培养所见的模式。提示IM患者外周血淋巴细胞中含有EB病毒，是一种非生产性未表达的感染，在体外被激活产生病毒，释放的颗粒感染邻近细胞形成转化株。我们考虑了这一机制与Burkitt淋巴瘤恶性细胞在培养中长出线条的不同之处，并讨论了它们的意义。
Transformation to continuous cell lines has been studied in cultures of peripheral leukocytes from infectious mononucleosis (IM) patients and in co‐cultures of IM leukocytes and foetal cord blood leukocytes of opposite sex. The transformed cells in the co‐cultures were of mixed origin with foetal cells usually predominating. Neutralizing antisera to EB virus markedly reduced or abolished the incidence of transformation in IM leukocyte cultures. This effect was not due to cytotoxicity and followed the pattern seen with cultures where transformation was known to depend on the intercellular transfer of infectious EB virus. The findings suggest that EB virus is harboured in peripheral lymphocytes of IM patients as a non‐productive unexpressed infection which is activated to produce virus in vitro, the particles released then infecting neighbouring cells to give transformed lines. The differences between this mechanism and the one whereby lines arise in culture from the malignant cells of Burkitt's lymphoma are considered, and their significance is discussed.