Protein arginine methyltransferase 1 mediates regeneration in Dugesia japonica
Protein arginine methyltransferase 1 mediates regeneration in Dugesia japonica
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蛋白质精氨酸甲基转移酶 1 介导 Dugesia japonica 的再生
DOI：
10.1016/j.bbrc.2020.01.087
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发表时间：
2020
影响因子：
3.1
通讯作者：
Pang Qiuxiang
中科院分区：
文献类型：
--
作者：
Zhang Yichao;Wu Weiwei;Gao Lili;Chen Meishan;Liu Xi;Huang Mujie;Li Ao;Zheng Kang;Liu Dongwu;Deng Hongkuan;Zhao Bosheng;Liu Baohua;Pang Qiuxiang
作为典型的扁形动物，日本三角洲（Dugesia japonica）因其强大的再生能力而受到越来越多的关注。蛋白质精氨酸甲基转移酶（PRMT）家族是一类具有甲基转移酶活性的酶，在体内许多重要的生理过程中发挥着重要作用。PRMT 1是一种优势的Ⅰ型PRMT，已报道在地中海施米德茶中表达。然而，PRMT 1基因在日本三角涡虫中的存在及其生物学功能还有待进一步研究。本研究获得了DjPRMT 1的全长序列，证实其为一个保守蛋白。全膜原位杂交结果显示，DjPRMT 1主要表达于蠕虫的新生母细胞中，并在再生早期的伤口部位有明显的聚集。DjPRMT 1基因的沉默延缓了涡虫的运动，减少了DjPIWI-A的表达，并且DjPRMT 1敲低也影响了涡虫的再生，在后向伤口再生的芽基周围的增殖略有减弱。这些初步结果表明DjPRMT 1参与了真涡虫的再生。
As a typical organism of platyhelminth,Dugesia japonicaattracts more and more attention for its strong regenerative ability. Protein arginine methyltransferase (PRMT) family is composed of a class of enzymes with methyltransferase activities, which play fundamental roles in vivo in many important physiological processes. PRMT1 is a predominant type Ⅰ PRMT, which has been reported to be expressed inSchmidtea mediterranea. Nevertheless, the existence and the specific biological functions of PRMT1 inDugesia japonicaneed further investigation. In this study, we acquired the full-length sequence ofDjPRMT1 and confirmed it was a conserved protein. Thereafter, whole-mountin situhybridization results showedDjPRMT1was mainly expressed in neoblasts of adult worms, and obvious aggregation ofDjPRMT1was observed at the wound site in early stages of regeneration. Silencing of theDjPRMT1gene retarded the movement of planarians with decreasedDjPIWI-Aexpression, andDjPRMT1knockdown also affected planarian regeneration with slightly attenuated proliferation around the blastema of posterior-facing wounds regeneration. In summary, these preliminary results demonstratedDjPRMT1was involved in the regeneration of planarian.