Arsenic biotransformation in industrial wastewater treatment residue: Effect of co-existing Shewanella sp. ANA-3 and MR-1
Arsenic biotransformation in industrial wastewater treatment residue: Effect of co-existing Shewanella sp. ANA-3 and MR-1
复制标题
工业废水处理残渣中砷的生物转化：共存希瓦氏菌的影响。
DOI：
10.1016/j.jes.2021.08.037
复制
发表时间：
2022
影响因子：
6.9
通讯作者：
Jing Chuanyong
中科院分区：
文献类型：
--
作者：
Li Hao;Zhang Lixin;Ye Li;Jing Chuanyong
具有呼吸性砷酸盐还原酶（ArrAB）的希瓦氏菌ANA-3和具有铁还原能力的MR-1在高砷废渣中共存。然而，它们对As转化和流动性的协同影响仍不清楚。为了确定哪种细菌，ANA-3或MR-1，占主导地位的流动性共存的环境中，我们探讨了希瓦氏菌属ANA-3和MR-1的存在下，在工业废渣中的生物转化。培养结果表明，As（III）是该菌的主要可溶性形态，ANA-3菌株对As的迁移起主导作用。ANA-3的作用被MR-1减弱，可能是由于这两种细菌之间的生存竞争。显微X射线荧光和X射线光电子能谱分析结果进一步揭示了ANA-3增强As迁移率的途径。菌株ANA-3几乎100%还原表面结合的Fe（III），并因此导致As（V）释放。溶解的As（V）然后被ANA-3还原为As（III）。这项研究的结果有助于了解砷在地下的命运，并强调了安全处置高含砷工业废物的重要性。
Shewanella sp. ANA-3 with the respiratory arsenate reductase (ArrAB) and MR-1 with ferric reduction ability always coexist in the presence of high arsenic (As)-containing waste residue. However, their synergistic impacts on As transformation and mobility remain unclear. To identify which bacterium, ANA-3 or MR-1, dominates As mobility in the coexisting environment, we explored the As biotransformation in the industrial waste residue in the presence of Shewanella sp. ANA-3 and MR-1. The incubation results show that As(III) was the main soluble species, and strain ANA-3 dominated As mobilization. The impact of ANA-3 was weakened by MR-1, probably due to the survival competition between these two bacteria. The results of micro X-ray fluorescence and X-ray photoelectron spectroscopy analyses further reveal the pathway for ANA-3 to enhance As mobility. Strain ANA-3 almost reduced 100% surface-bound Fe(III), and consequently led to As(V) release. The dissolved As(V) was then reduced to As(III) by ANA-3. The results of this study help to understand the fate of arsenic in the subsurface and highlight the importance of the safe disposal of high As-containing industrial waste.