Genetic evidence suggests that Spata22 is required for the maintenance of Rad51 foci in mammalian meiosis.
Genetic evidence suggests that Spata22 is required for the maintenance of Rad51 foci in mammalian meiosis.
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DOI：
10.1038/srep06148
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发表时间：
2014-08-21
影响因子：
4.6
通讯作者：
Kitada K
中科院分区：
文献类型：
--
作者：
Ishishita S;Matsuda Y;Kitada K
减数分裂结节是双链DNA断裂修复的位点。 Rpa是一种单链DNA结合蛋白，Rad51是一种协助修复DNA双链断裂的蛋白。在小鼠体内 Rpa 定位之前，Rad51 定位到减数分裂结节，这引入了 Rpa 是否参与哺乳动物减数分裂过程中突触前丝形成的问题。在这里，我们发现一种功能未知的蛋白质 Spata22 与 Rpa 共定位于大鼠精母细胞减数分裂结节中。在 Spata22 缺陷突变大鼠的精母细胞中，减数分裂在合子期样阶段被抑制，并且在细线期和合子期样阶段观察到正常数量的 Rpa 灶。 Rad51 灶的数量最初正常，但从细线期开始下降。这些结果表明，Rpa 灶点的形成和维持均独立于 Spata22，Rad51 灶点的维持而非形成需要 Spata22。我们提出了哺乳动物减数分裂中突触前丝形成的可能模型，其中涉及 Rpa 和 Spata22。
Meiotic nodules are the sites of double-stranded DNA break repair. Rpa is a single-stranded DNA-binding protein, and Rad51 is a protein that assists in the repair of DNA double strand breaks. The localisation of Rad51 to meiotic nodules before the localisation of Rpa in mice introduces the issue of whether Rpa is involved in presynaptic filament formation during mammalian meiosis. Here, we show that a protein with unknown function, Spata22, colocalises with Rpa in meiotic nodules in rat spermatocytes. In spermatocytes of Spata22-deficient mutant rats, meiosis was arrested at the zygotene-like stage, and a normal number of Rpa foci was observed during leptotene- and zygotene-like stages. The number of Rad51 foci was initially normal but declined from the leptotene-like stage. These results suggest that both formation and maintenance of Rpa foci are independent of Spata22, and the maintenance, but not the formation, of Rad51 foci requires Spata22. We propose a possible model of presynaptic filament formation in mammalian meiosis, which involves Rpa and Spata22.
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