Identification of biomarkers associated with metabolic cardiovascular disease using mRNA-SNP-miRNA regulatory network analysis.
Identification of biomarkers associated with metabolic cardiovascular disease using mRNA-SNP-miRNA regulatory network analysis.
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DOI：
10.1186/s12872-021-02166-4
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发表时间：
2021-07-23
影响因子：
2.1
通讯作者：
Liu K
中科院分区：
文献类型：
--
作者：
Fan Z;Peng W;Wang Z;Zhang L;Liu K
CVD是T2 DM患者死亡的主要原因。然而，在T2 DM的早期阶段，很少有生物标志物被鉴定用于检测和诊断CVD。本研究的目的是鉴定与代谢性心血管疾病相关的重要mRNA、微RNA和单核苷酸多态性（SNP）。表达谱和GWAS数据从Gene ExpressionOmnibus（GEO）数据库获得。通过Illumina HiSeq 2000平台在T2 DM患者和T2 DM伴CVD患者中进行miRNA测序。进行EQTL分析和基因本体（GO）、京都基因和基因组百科全书（KEGG）途径富集分析。利用Cytoscape 3.7.2软件建立MRNA-miRNA共表达网络和mRNA-SNP-miRNA相互作用网络。在我们的研究中，我们确定了56个基因和16个miRNAs的显着差异表达。KEGG分析结果表明，差异表达基因的生物学功能包括B细胞受体信号通路和造血细胞谱系。MRNA-miRNA共表达网络和mRNA-SNP-miRNA相互作用网络表明let-7i-5 p、RASGRP 3、KRT 1和CEP 41可能是T2 DM患者CVD早期检测和诊断的潜在生物标志物。我们的研究结果表明，下调let-7i-5 p，上调RASGRP 3，KRT 1和CEP 41可能在T2 DM患者CVD发生和发展的分子机制中起关键作用。在线版本包含补充材料，可通过10.1186/s12872-021-02166-4获得。
CVD is the leading cause of death in T2DM patients. However, few biomarkers have been identified to detect and diagnose CVD in the early stage of T2DM. The aim of our study was to identify the important mRNAs, micro (mi)RNAs and SNPs (single nucleotide polymorphisms) that are associated with metabolic cardiovascular disease. Expression profiles and GWAS data were obtained from Gene Expression Omnibus (GEO) database. MiRNA-sequencing was conducted by Illumina HiSeq 2000 platform in T2DM patients and T2DM with CVD patients. EQTL analysis and gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were conducted. MRNA-miRNA co-expression network and mRNA-SNP-miRNA interaction network were established and visualized by Cytoscape 3.7.2. In our study, we identified 56 genes and 16 miRNAs that were significantly differentially expressed. KEGG analyses results indicated that B cell receptor signaling pathway and hematopoietic cell lineage were included in the biological functions of differentially expressed genes. MRNA-miRNA co-expression network and mRNA-SNP-miRNA interaction network illustrated that let-7i-5p, RASGRP3, KRT1 and CEP41 may be potential biomarkers for the early detection and diagnosis of CVD in T2DM patients. Our results suggested that downregulated let-7i-5p, and upregulated RASGRP3, KRT1 and CEP41 may play crucial roles in molecular mechanisms underlying the initiation and development of CVD in T2DM patients. The online version contains supplementary material available at 10.1186/s12872-021-02166-4.
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影响因子：
64.8
作者：
通讯作者：
--
影响因子：
120.7
作者：
Qi, Lu;Qi, Qibin;Prudente, Sabrina;Mendonca, Christine;Andreozzi, Francesco;di Pietro, Natalia;Sturma, Mariella;Novelli, Valeria;Mannino, Gaia Chiara;Formoso, Gloria;Gervino, Ernest V.;Hauser, Thomas H.;Muehlschlegel, Jochen D.;Niewczas, Monika A.;Krolewski, Andrzej S.;Biolo, Gianni;Pandolfi, Assunta;Rimm, Eric;Sesti, Giorgio;Trischitta, Vincenzo;Hu, Frank;Doria, Alessandro
通讯作者：
Doria, Alessandro
影响因子：
16.6
作者：
Caporali A;Meloni M;Nailor A;Mitić T;Shantikumar S;Riu F;Sala-Newby GB;Rose L;Besnier M;Katare R;Voellenkle C;Verkade P;Martelli F;Madeddu P;Emanueli C
通讯作者：
Emanueli C
影响因子：
7.7
作者：
King GL;Park K;Li Q
通讯作者：
Li Q
影响因子：
64.5
作者：
Lewis, BP;Shih, IH;Burge, CB
通讯作者：
Burge, CB