Noninvasive ultrasound imaging of inflammation using microbubbles targeted to activated leukocytes
Noninvasive ultrasound imaging of inflammation using microbubbles targeted to activated leukocytes
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DOI：
10.1161/01.cir.102.22.2745
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发表时间：
2000-11-28
期刊：
影响因子：
37.8
通讯作者：
Kaul, S
中科院分区：
文献类型：
--
作者：
Lindner, JR;Song, J;Kaul, S
背景-用于超声灌注成像的脂质微泡保留在炎症组织中，因为补体介导的微泡附着在小静脉内的白细胞上。我们假设，将磷脂酰丝氨酸(PS)掺入微泡外壳可以通过放大补体激活来增强这些相互作用，从而允许炎症的超声成像。方法和结果-在6只小鼠中，对经组织坏死因子α处理的提睾肌进行活体显微镜观察，以评估外壳中有和没有PS的荧光素标记的脂肪微泡的微血管行为。在静脉注射10分钟后，含有PS的微泡附着在炎症小静脉内的白细胞比标准脂质微泡更多(每20个光场分别为20+/-4和10+/-3，P
Background-Lipid microbubbles used for perfusion imaging with ultrasound are retained within inflamed tissue because of complement-mediated attachment to leukocytes within venules. We hypothesized that incorporation of phosphatidylserine (PS) into the microbubble shell may enhance these interactions by amplifying complement activation and thereby allow ultrasound imaging of inflammation.Methods and Results-In 6 mice, intravital microscopy of tissue necrosis factor-alpha -treated cremaster muscle was performed to assess the microvascular behavior of fluorescein-labeled lipid microbubbles with and without PS in the shell. Ten minutes after intravenous injection, microbubble attachment to leukocytes within inflamed venules was greater for PS-containing than for standard lipid microbubbles (20+/-4 versus 10+/-3 per 20 optical fields, P