Hybrid Paper-Plastic Microchip for Flexible and High-Performance Point-of-Care Diagnostics
Hybrid Paper-Plastic Microchip for Flexible and High-Performance Point-of-Care Diagnostics
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DOI：
10.1002/adfm.201707161
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发表时间：
2018-06-27
影响因子：
19
通讯作者：
Shafiee, Hadi
中科院分区：
文献类型：
--
作者：
Draz, Mohamed Shehata;Moazeni, Maryam;Shafiee, Hadi
低成本和易于制造的微芯片仍然是开发真正的医疗点(POC)诊断的关键挑战。纤维素纸和塑料是薄、轻、灵活和丰富的原材料，这使它们成为批量生产POC设备的极佳基板。为此，研制了一种纸塑混合微芯片(PPMC)，该芯片既可用于对不同目标的单一检测，也可用于对不同目标的多路检测，为微芯片的设计和制造提供了灵活性。对开发的带有印刷电子设备的PPMC进行了评估，以敏感和可靠地检测广泛的靶标，如肝癌和结肠癌蛋白生物标记物、完整的寨卡病毒和人乳头状瘤病毒核酸扩增。所提出的方法允许对被测目标进行高度特异性的检测，对蛋白质生物标记物的检测下限为10(2)ng mL(-1)，对病毒颗粒的检测下限为10(3)粒子/毫升，对于目标核酸的检测下限为10(2)拷贝/微升。这种方法可能被考虑用于开发廉价和稳定的POC微芯片诊断，并适用于广泛的微生物感染和癌症生物标志物的检测。
A low-cost and easy-to-fabricate microchip remains a key challenge for the development of true point-of-care (POC) diagnostics. Cellulose paper and plastic are thin, light, flexible, and abundant raw materials, which make them excellent substrates for mass production of POC devices. Herein, a hybrid paper-plastic microchip (PPMC) is developed, which can be used for both single and multiplexed detection of different targets, providing flexibility in the design and fabrication of the microchip. The developed PPMC with printed electronics is evaluated for sensitive and reliable detection of a broad range of targets, such as liver and colon cancer protein biomarkers, intact Zika virus, and human papillomavirus nucleic acid amplicons. The presented approach allows a highly specific detection of the tested targets with detection limits as low as 10(2) ng mL(-1) for protein biomarkers, 10(3) particle per milliliter for virus particles, and 10(2) copies per microliter for a target nucleic acid. This approach can potentially be considered for the development of inexpensive and stable POC microchip diagnostics and is suitable for the detection of a wide range of microbial infections and cancer biomarkers.