Immunohistochemical analysis of the different neuropeptide containing nerve fibres and immunocompetent cells in the gastritis
Immunohistochemical analysis of the different neuropeptide containing nerve fibres and immunocompetent cells in the gastritis
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胃炎中含有神经纤维和免疫活性细胞的不同神经肽的免疫组织化学分析
DOI：
10.1055/s-2004-827036
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
E. Fehér
中科院分区：
文献类型：
--
作者：
G. Sipos;B. Batbayar;K. Altdorfer;E. Fehér
方法：采用ABC免疫组化方法检测血管活性肠多肽（VIP）、神经肽Y （NPY）、P物质（SP）、一氧化氮合酶（NOS）和酪氨酸β-羟化酶（TH） IR神经元件和多（adp -核糖）聚合酶（PARP活化指标）IR结缔组织细胞。结果：对不同神经肽含量的神经纤维密度进行定量分析，发现粘膜中存在不同密度的IR神经纤维。NPY和thir神经纤维主要分布在血管周围，腺体周围仅见少量。一些红外神经纤维也被发现与免疫细胞密切相关。胃炎活检材料中NPY IR神经纤维数量略减少，而SP、VIP和TH IR神经末梢数量显著增加（p< 0.001）。部分免疫活性细胞对SP和NPY也表现出IR。胃炎组PARP IR细胞明显增多（p< 0.001）。结论：IR神经纤维密度的改变和PARP的激活可能在炎症急性期和胃炎的发病机制中起作用。
Methods: Vasoactive intestinal polypeptide (VIP), neuropeptide Y (NPY), substance P (SP), nitric oxide synthase (NOS) and tyrosine β-hydroxylase (TH) IR nerve elements and poly (ADP-ribose) polymerase (indicator of PARP activation) IR connective tissue cells were detected by ABC immunohistochemistry.Results: The quantitative analysis of the density of the different neuropeptide containing nerve fibres showed that the IR nerve fibres were found in the mucosa with different density. The NPY and TH IR nerve fibres were mainly located around the blood vessels and only a few of them were observed around the glands. Some IR nerve fibres were also found in a close association to the immunocytes. In the biopsy materials of gastritis the number of NPY IR nerve fibres was slightly decreased, however, the number of SP, VIP and TH IR nerve terminals was significantly (p< 0.001) increased. Some immunocompetent cells showed also IR for SP and NPY. Significantly (p< 0.001) increased PARP IR cells were detected in the gastritis.Conclusions: The changes of the density of IR nerve fibres and PARP activation might play a role in the acute phase of inflammation as well as in the pathogenesis of gastritis.