Characterization of paired Ig-like receptors in rats.
Characterization of paired Ig-like receptors in rats.
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DOI：
10.4049/jimmunol.163.12.6371
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发表时间：
1999-12
影响因子：
4.4
通讯作者：
G. Dennis;R. Stephan;H. Kubagawa;M. Cooper
中科院分区：
文献类型：
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作者：
G. Dennis;R. Stephan;H. Kubagawa;M. Cooper
为了探索激活型 (PIR-A) 和抑制型 (PIR-B) 型小鼠配对 Ig 样受体的系统发育史，我们从大鼠脾细胞 cDNA 文库中分离了 PIR 同源物。大鼠 (ra) PIR-A 和 raPIR-B cDNA 序列预测具有六个高度保守的细胞外 Ig 样结构域和独特的近膜区、跨膜区和细胞质区的跨膜蛋白。 raPIR-B 细胞质区域包含原型抑制基序，而 raPIR-A 具有带电跨膜区域和短细胞质尾部。 Southern 印迹分析预测大鼠基因组中存在多个 Pira 基因和单个 Pirb 基因。尽管 raPIR-A 和 raPIR-B 由骨髓细胞协调表达，但 mRNA 分析检测到 B 细胞中的 raPIR-A 和 NK 细胞中的 raPIR-B 表达不成对。总的来说，这些发现表明 Pir 基因家族的结构特征在大鼠和小鼠中是保守的，但表明 PIR 调控元件在啮齿类动物物种形成过程中存在差异。
To explore the phylogenetic history of the murine paired Ig-like receptors of activating (PIR-A) and inhibitory (PIR-B) types, we isolated PIR homologues from a rat splenocyte cDNA library. The rat (ra) PIR-A and raPIR-B cDNA sequences predict transmembrane proteins with six highly conserved extracellular Ig-like domains and distinctive membrane proximal, transmembrane, and cytoplasmic regions. The raPIR-B cytoplasmic region contains prototypic inhibitory motifs, whereas raPIR-A features a charged transmembrane region and a short cytoplasmic tail. Southern blot analysis predicts the presence of multiple Pira genes and a single Pirb gene in the rat genome. Although raPIR-A and raPIR-B are coordinately expressed by myeloid cells, analysis of mRNA detected unpaired expression of raPIR-A by B cells and raPIR-B by NK cells. Collectively, these findings indicate that the structural hallmarks of the Pir gene family are conserved in rats and mice, yet suggest divergence of PIR regulatory elements during rodent speciation.