Gender-related differences in beta-adrenergic receptor-mediated cardiac remodeling
Gender-related differences in beta-adrenergic receptor-mediated cardiac remodeling
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β-肾上腺素能受体介导的心脏重塑中的性别相关差异
DOI：
10.1139/cjpp-2016-0103
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发表时间：
2016
影响因子：
2.1
通讯作者：
Li Zijian
中科院分区：
文献类型：
--
作者：
Zhu Baoling;Liu Kai;Yang Chengzhi;Qiao Yuhui;Li Zijian
心脏重构是各种心血管疾病的病理基础。在这项研究中，我们发现β-肾上腺素能受体（AR）介导的病理性心脏重构存在性别差异。皮下注射异丙肾上腺素（ISO）14天建立心脏重构模型。在ISO给药期间的第7天和第14天测量心率（HR）、平均动脉压（MAP）和超声心动图。检测心肌横截面积和心脏质量/胫骨长度比值（HM/TL），评价心肌肥厚程度。苦味酸-天狼星红染色（苦味酸+天狼星红F3 B）用于评价心脏纤维化。心肌毛细血管密度通过血管性血友病因子的免疫组织化学评估。实时荧光定量PCR检测β1-AR和β2-AR的表达。结果表明，异丙肾上腺素可诱导小鼠心脏重构，且其程度在雌雄之间存在差异。女性心脏壁厚度、心肌横截面积和胶原沉积的增加程度小于男性。然而，在HR、MAP、心功能和心肌毛细血管密度方面未观察到性别相关差异。β2-AR表达的显著降低，而不是β1-AR表达的降低，似乎导致了心脏重塑的性别相关差异。
Cardiac remodeling is the pathological basis of various cardiovascular diseases. In this study, we found gender-related differences in β-adrenergic receptor (AR)-mediated pathological cardiac remodeling. Cardiac remodeling model was established by subcutaneous injection of isoprenaline (ISO) for 14 days. Heart rate (HR), mean arterial pressure (MAP), and echocardiography were obtained on 7th and 14th days during ISO administration. Myocardial cross-sectional area and the ratio of heart mass to tibia length (HM/TL) were detected to assess cardiac hypertrophy. Picro–Sirius red staining (picric acid + Sirius red F3B) was used to evaluate cardiac fibrosis. Myocardial capillary density was assessed by immunohistochemistry for von Willebrand factor. Further, real-time PCR was used to measure the expression of β1-AR and β2-AR. Results showed that ISO induced cardiac remodeling, the extent of which was different between female and male mice. The extent of increase in cardiac wall thickness, myocardial cross-sectional area, and collagen deposition in females was less than that in males. However, no gender-related difference was observed in HR, MAP, cardiac function, and myocardial capillary density. The distinctive decrease of β2-AR expression, rather than a decrease of β1-AR expression, seemed to result in gender-related differences in cardiac remodeling.