Effect of acute exercise on RBC deformability and RBC nitric oxide synthase signalling pathway in young sickle cell anaemia patients
Effect of acute exercise on RBC deformability and RBC nitric oxide synthase signalling pathway in young sickle cell anaemia patients
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DOI：
10.1038/s41598-019-48364-1
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发表时间：
2019-08-14
影响因子：
4.6
通讯作者：
Prokop, Aram
中科院分区：
文献类型：
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作者：
Grau, Marijke;Jerke, Max;Prokop, Aram
镰状细胞性贫血（SCA）的特征在于降低的红细胞（RBC）变形性和一氧化氮（NO）生物利用度。这项研究的目的是调查是否运动可能会影响这些参数在SCA。SCA患者和健康对照组（AA）进行急性次极量运动试验，直至受试者达到第一阈（VT 1）。在静息和VT 1时采集血液样本。在休息时，游离血红蛋白水平较高，红细胞计数，血红蛋白和红细胞压积较低的SCA相比，AA。SCA组红细胞变形能力较低。运动对测试参数没有影响。与AA相比，SCA中安静时RBC NO水平较高，而SCA中运动后RBC NO水平显着下降。这可能与红细胞一氧化氮合酶（RBC-NOS）的活性降低有关，而这种活性仅在运动后SCA中观察到。自由基水平较高的SCA在休息，但浓度不受运动。SCA和AA的脂质过氧化和抗氧化能力指标相似，不受运动的影响。总之，一个单一的急性次极量运动对SCA的RBC变形性或氧化应激标志物没有有害影响，似乎调节RBC-NOS信号通路。
Sickle cell anaemia (SCA) is characterized by reduced red blood cell (RBC) deformability and nitric oxide (NO) bioavailability. The aim of the study was to investigate whether exercise might affect these parameters in SCA. SCA patients and healthy controls (AA) performed an acute submaximal exercise test until subjects reached the first ventilatory threshold (VT1). Blood was sampled at rest and at VT1. At rest, free haemoglobin level was higher and RBC count, haemoglobin and haematocrit were lower in SCA compared to AA. RBC deformability was lower in SCA. Exercise had no effect on the tested parameters. RBC NO level was higher in SCA compared to AA at rest and significantly decreased after exercise in SCA. This might be related to a reduction in RBC-NO synthase (RBC-NOS) activation which was only observed in SCA after exercise. Free radical levels were higher in SCA at rest but concentration was not affected by exercise. Marker for lipid peroxidation and antioxidative capacity were similar in SCA and AA and not affected by exercise. In conclusion, a single acute submaximal bout of exercise has no deleterious effects on RBC deformability or oxidative stress markers in SCA, and seems to modulate RBC-NOS signalling pathway.