Association of specific cytomegalovirus genotypes with death from myelosuppression after marrow transplantation
Association of specific cytomegalovirus genotypes with death from myelosuppression after marrow transplantation
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DOI：
10.1182/blood.v90.5.2097
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发表时间：
1997-09-01
期刊：
影响因子：
20.3
通讯作者：
Gooley, T
中科院分区：
文献类型：
--
作者：
TorokStorb, B;Boeckh, M;Gooley, T
对281株来自骨髓移植受者的巨细胞病毒(CMV)进行了基因分型。基因分型基于编码包膜糖蛋白B(GB)基因的序列变异，通过聚合酶链式反应(PCR)扩增的GB DNA的限制性内切酶分析检测到。在所有被研究的菌株中，GB型1-4型的分布分别为48.4%、16.4%、24.6%和8.2%，只有2.5%的菌株含有一种以上的gB型。除GB类型外，协变量还包括潜在疾病类型、供受者人类白细胞抗原配型、供者巨细胞病毒血清状态和作为连续变量的年龄。与骨髓抑制相关的死亡率分别为2.9%或4/136例，0%或0/46例，21.7%或15/69例，17.4%或4/23例。多因素分析显示巨细胞病毒gB亚型与骨髓抑制死亡显著相关(P
A retrospective analysis of cytomegalovirus (CMV) genotype was conducted on 281 CMV isolates obtained from marrow transplant recipients. The genotyping was based on sequence variations in the gene encoding envelope glycoprotein B (gB) as detected by restriction analysis of polymerase chain reaction (PCR)-amplified gB DNA. Among all isolates studied, the distribution of gB types 1-4 was 48.4%, 16.4 %, 24.6%, and 8.2%, respectively, with only 2.5% of all isolates containing more than one gB type, The association of gB types with acute graft-versus-host-disease (GVHD) and death related to myelosuppression was examined using appropriate multivariable regression models. Covariables in addition to gB type included underlying disease type, donor-recipient HLA matching, donor CMV serostatus, and age as a continuous variable. Death associated with myelosuppression occurred in 2.9% or 4 of 136 patients with gB1, 0% or 0 of 46 patients with gB2, 21.7% or 15 of 69 patients with gB3, and 17.4% or four of 23 patients with gB4. The significant association of CMV gB type with death due to myelosuppression was maintained in a multivariable analysis (P