Structural characterization of the O-specific polysaccharide from the lipopolysaccharide of the fish pathogen Aeromonas bestiarum strain P1S
Structural characterization of the O-specific polysaccharide from the lipopolysaccharide of the fish pathogen Aeromonas bestiarum strain P1S
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DOI：
10.1016/j.carres.2011.02.003
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发表时间：
2011-05-01
影响因子：
3.1
通讯作者：
Holst, Otto
中科院分区：
文献类型：
--
作者：
Turska-Szewczuk, Anna;Guz, Leszek;Holst, Otto
采用糖基分析、甲基化分析、沿着核磁共振氢谱和碳谱等方法对牛斯气单胞菌PIS的脂多糖进行了研究。使用（1）H，（1）H NOESY和（1）H，（13）C HMBC实验确定糖残基的序列。发现O-特异性多糖是由结构为-> 2）-β-D-Quip 3 NAcyl-（1 -> 2）-β-D-Ribf-（1 -> 4）-β-D-Galp-（1 -> 3）-α-D-FucpNAc-（1 -> 4）的四糖重复单元组成的高分子量多糖。假定所阐明的结构也代表O-特异性多糖的生物重复单元。(C)2011爱思唯尔有限公司保留所有权利。
The O-specific polysaccharide obtained by mild-acid degradation of lipopolysaccharide of Aeromonas bestiarum PIS was studied by sugar and methylation analyses along with (1)H and (13)C NMR spectroscopy. The sequence of the sugar residues was determined using (1)H,(1)H NOESY and (1)H,(13)C HMBC experiments. The O-specific polysaccharide was found to be a high-molecular-mass polysaccharide composed of tetrasaccharide repeating units of the structure-> 2)-beta-D-Quip3NAcyl-(1 -> 2)-beta-D-Ribf-(1 -> 4)-beta-D-Galp-(1 -> 3)-alpha-D-FucpNAc-(1 ->Since small amounts of a terminal Quip3N residue were identified in methylation analysis, it was assumed that the elucidated structure also represented the biological repeating unit of the O-specific polysaccharide. (C) 2011 Elsevier Ltd. All rights reserved.