Comparative transcriptional analyses of Pleurotus ostreatus mutants on beech wood and rice straw shed light on substrate-biased gene regulation
Comparative transcriptional analyses of Pleurotus ostreatus mutants on beech wood and rice straw shed light on substrate-biased gene regulation
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DOI：
10.1007/s00253-020-11087-9
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发表时间：
2021-01
影响因子：
5
通讯作者：
Hongli Wu;T. Nakazawa;Haibo Xu;Rui-Heng Yang;D. Bao;Moriyuki Kawauchi;M. Sakamoto;Y. Honda
中科院分区：
文献类型：
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作者：
Hongli Wu;T. Nakazawa;Haibo Xu;Rui-Heng Yang;D. Bao;Moriyuki Kawauchi;M. Sakamoto;Y. Honda
不同的木材降解者占据他们的首选栖息地，具有偏向的酶系，很好地适应他们的定居底物。平菇，通常发现在木材上，已经进化出自己的产酶特性。在我们之前的研究中，几个P。对包括Δhir1和Δgat1在内的ostreatus脱木素缺陷突变体进行了分析，结果表明，与其亲本菌株20b相比，在山毛榉木屑上木质素降解基因表达下调，而木质素降解基因和木聚糖降解基因表达上调。在这项研究中，稻草(RS)被用作替代底物来检测OFP的转录反应。在不同的底物上。20b株的evp1基因和一个铜还蛋白编码基因在RS上的表达显著高于在BWS上的表达，反映了它们不同的调控模式。编码葡萄糖醛酸酶的基因在RS上的总体表达水平也高于在BWS上的表达水平，与底物组成有很好的相关性。突变体(Δhir1或Δgat1对20b株)在RS上的转录变化与在BWS上的相似，并且突变体在RS上的胞外木质纤维素降解酶活性和木质素降解能力与相应的酶编码基因的转录变化一致。然而，与生长在BWS上的突变菌株相比，生长在RS上的突变菌株中属于同一CAZyme家族的特定编码酶基因的转录呈现出不同的变化模式。这些发现为木质细胞分解基因INP转录调控的分子机制提供了新的见解。Ostreatus。Key Points·P.ostreatus根据不同的底物表达不同的酶谱系相关基因。·木质素分解缺陷突变体似乎存在上调纤维素分解基因的需求。·某些特定基因的调控可能由需求驱动，取决于底物。
Distinct wood degraders occupying their preferred habitats have biased enzyme repertoires that are well fitted to their colonized substrates.Pleurotus ostreatus, commonly found on wood, has evolved its own enzyme-producing traits. In our previous study, transcriptional shifts in severalP. ostreatusdelignification-defective mutants, including Δhir1and Δgat1strains, were analyzed, which revealed the downregulation of ligninolytic genes and the upregulation of cellulolytic and xylanolytic genes when compared to their parental strain 20b on beech wood sawdust medium (BWS). In this study, rice straw (RS) was used as an alternative substrate to examine the transcriptional responses ofP. ostreatusto distinct substrates. Thevp1gene and a cupredoxin-encoding gene were significantly upregulated in the 20b strain on RS compared with that on BWS, reflecting their distinct regulation patterns. The overall expression level of genes encoding glucuronidases was also higher on RS than on BWS, showing a good correlation with the substrate composition. Transcriptional alterations in the mutants (Δhir1or Δgat1versus 20b strain) on RS were similar to those on BWS, and the extracellular lignocellulose-degrading enzyme activities and lignin-degrading ability of the mutants on RS were consistent with the transcriptional alterations of the corresponding enzyme-encoding genes. However, transcripts of specific genes encoding enzymes belonging to the same CAZyme family exhibited distinct alteration patterns in the mutant strains grown on RS compared to those grown on BWS. These findings provide new insights into the molecular mechanisms underlying the transcriptional regulation of lignocellulolytic genes inP. ostreatus.Key Points•P. ostreatus expressed variable enzymatic repertoire-related genes in response to distinct substrates.•A demand to upregulate the cellulolytic genes seems to be present in ligninolysis-deficient mutants.•The regulation of some specific genes probably driven by the demand is dependent on the substrate.