Occurrence and spatial distribution of antibiotic resistance genes in the Bohai Sea and Yellow Sea areas, China
Occurrence and spatial distribution of antibiotic resistance genes in the Bohai Sea and Yellow Sea areas, China
复制标题
中国渤海和黄海地区抗生素耐药基因的出现及空间分布
DOI：
10.1016/j.envpol.2019.05.143
复制
发表时间：
2019-09-01
影响因子：
8.9
通讯作者：
Lin, Yichen
中科院分区：
文献类型：
--
作者：
Lu, Jian;Zhang, Yuxuan;Lin, Yichen
Bohai和黄海经济边缘的快速发展导致人们关注新兴的海洋环境污染。这项研究调查了波海和黄海地区抗生素耐药性基因（ARGS）的空间分布。从Bohai Sea，黄海和从Yalu河口到长江河的海岸线的主要城市进行了大规模采样。基于绝对丰富的目标ARG的空间分布在于河水沿海水的趋势> Bohai Sea>黄色海洋，近海>近海和内湾>海湾口。沿海水域中选定的ARG的总绝对丰度（1.23 x 10（4）-3.94 x 10（5）副本/ml）比海中的副本大约1-4个数量级（21.1.1-8.00 x 10（（ 3）副本/毫升）。在黄海和沿海地区，ARG的丰富性大大波动。磺酰胺耐药基因在Bohai和黄海中拥有最高的丰度（最高2.13 x 10（3）副本/ml静止的副本和6.23 x 10（3）副本/ml的SUL2），然后是四环素和喹诺酮类抗性基因，而奎诺酮抗性基因QNRA在沿海地区拥有最高的丰度（最高3.66 x 10（5）副本/毫升）。在大多数不同的水生系统中，沉积物和相应水样品之间靶基因的分布系数超过1.0。根据主要成分分析和冗余分析，从海洋中收集的水样聚集在一起，而沿海地区和河流则分开。铵和硝酸盐在ARGS的分布和变化中起着重要作用。共发生网络分析表明，在黄海中检测到潜在的多种抗生素抗生素抗生素的抗性细菌，其丰度高于Bohai Sea。这些观察结果对波海和黄海地区的ARG污染状况提供了全面的新见解。 （c）2019 Elsevier Ltd.保留所有权利。
Rapid development of Bohai and Yellow Sea Economic Rim has led to the concern of emerging contamination of marine environments. This study investigated the spatial distribution of antibiotic resistance genes (ARGs) in Bohai and Yellow Sea areas. A large scale sampling from Bohai Sea, Yellow Sea and the major cities along the coastline from the mouth of Yalu River to the Yangtze River was performed. The spatial distribution of target ARGs based on the absolute abundances was in the trend of river water coastal water > the Bohai Sea > the Yellow Sea, inshore > offshore and inner bay > bay mouth. The total absolute abundances of selected ARGs in the coastal waters (1.23 x 10(4)-3.94 x 10(5) copies/mL) were about 1-4 orders of magnitude higher than those in the sea (21.1-8.00 x 10(3) copies/mL). The abundances of ARGs fluctuated greatly in the Yellow Sea and the coastal areas. Sulfonamide resistance genes hold the highest abundances in the Bohai and Yellow Sea (up to 2.13 x 10(3) copies/mL of still and 6.23 x 10(3) copies/mL of sul2), followed by tetracycline and quinolone resistance genes, while qnrA hold the highest abundances in coastal areas (up to 3.66 x 10(5) copies/mL). The distribution coefficients of target genes between sediments and corresponding water samples were more than 1.0 in the majority of different aquatic systems. According to the principle component analysis and redundancy analysis, water samples collected from the sea clustered together while those from the coastal zone and rivers were separated. Ammonium and nitrate played important roles in the distribution and variation of ARGs. Co-occurrence network analysis revealed that the potential multi-antibiotics resistant bacteria were detected with higher abundances in the Yellow Sea than in the Bohai Sea. These observations provided a comprehensive new insight into the pollution status of ARGs in the Bohai and Yellow Sea areas. (C) 2019 Elsevier Ltd. All rights reserved.