Identification of sleep-promoting neurons in vitro
Identification of sleep-promoting neurons in vitro
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DOI：
10.1038/35010109
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发表时间：
2000-04-27
期刊：
影响因子：
64.8
通讯作者：
Serafin, M
中科院分区：
文献类型：
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作者：
Gallopin, T;Fort, P;Serafin, M
负责睡眠开始的神经元被认为位于视前区（1-3），更具体地说，位于腹外侧视前核（VLPO）（4-6）。在这里，我们在体外识别出促进睡眠的神经元，并表明它们代表了一个同质的细胞群体，在清醒状态下必须被唤醒系统抑制。我们发现三分之二的VLPO神经元是多极三角形细胞，表现出低阈值的棘波。这一比例与同一区域睡眠期间活跃的细胞比例相匹配（6）。然后，我们使用单细胞逆转录酶和聚合酶链反应表明，这些神经元可能含有g-氨基丁酸（GABA）。我们还发现，这些神经元受到去甲肾上腺素和乙酰胆碱的抑制，两者都是觉醒的递质（3，7，8）。由于这些细胞中的大多数也受到5-羟色胺的抑制，但不受组胺的影响，因此觉醒递质对它们的总体抑制与它们在清醒时相对于睡眠时的相对不活动性一致（6）。我们提出，这种VLPO神经元与它们投射到的去甲肾上腺素能、胆碱能和胆碱能觉醒系统的相互抑制相互作用（5，9，10）是促进睡眠的关键因素。
The neurons responsible for the onset of sleep are thought to be located in the preoptic area(1-3) and more specifically, in the ventrolateral preoptic nucleus (VLPO)(4-6). Here we identify sleep-promoting neurons in vitro and show that they represent an homogeneous population of cells that must be inhibited by systems of arousal during the waking state. We rnd that two-thirds of the VLPO neurons are multipolar triangular cells that show a low-threshold spike. This proportion matches that of cells active during sleep in the same region(6). We then show, using single-cell reverse transcriptase followed by polymerase chain reaction, that these neurons probably contain g-aminobutyric acid (GABA). We also show that these neurons are inhibited by noradrenaline and acetylcholine, both of which are transmitters of wakefulness(3,7,8). As most of these cells are also inhibited by serotonin but unaffected by histamine, their overall inhibition by transmitters of wakefulness is in agreement with their relative inactivity during waking with respect to sleep(6). We propose that the reciprocal inhibitory interaction of such VLPO neurons with the noradrenergic, serotoninergic and cholinergic waking systems to which they project(5,9,10) is a key factor for promoting sleep.