Multi-faceted integrated omics analysis revealed parsley (Petroselinum crispum) as a novel dietary intervention in dextran sodium sulphate induced colitic mice
Multi-faceted integrated omics analysis revealed parsley (Petroselinum crispum) as a novel dietary intervention in dextran sodium sulphate induced colitic mice
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DOI：
10.1016/j.jff.2014.09.018
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发表时间：
2014-11-01
影响因子：
5.6
通讯作者：
Kato, Hisanori
中科院分区：
文献类型：
--
作者：
Jia, Huijuan;Aw, Wanping;Kato, Hisanori
在这里，我们描述了我们前所未有的方法，通过使用葡聚糖硫酸钠（DSS）诱导的结肠炎进行多组学评估，提出欧芹作为炎症性肠病（IBD）的营养干预。给7周龄雄性C57 BL/6 J小鼠喂食2%欧芹或基础饮食并饮用正常饮用水1周，之后通过施用1.5%（w/v）DSS饮用水9天诱导结肠炎。欧芹补充剂显著改善结肠缩短和增加疾病活动指数。结肠转录组显示炎性细胞因子下调，肝转录组和代谢组显示脂肪酸合成基因上调，从而改善体重减轻。在肝转录组和蛋白质组中观察到下调的癌症标志物。血浆代谢物分析表明，柠檬酸和尿素循环的变化，涉及改善受损的糖酵解和氧化应激。欧芹在预防IBD中的作用被这一开创性的多整合组学所强调，该多整合组学预计将是有用的和信息丰富的，并塑造营养保健品和功能性食品研究的未来。(C)2014作者由Elsevier出版
Here we describe our unprecedented approach in proposing parsley as a nutraceutical intervention in inflammatory bowel disease (IBD) via multi-omics evaluation using dextran sodium sulphate (DSS)-induced colitis. Seven-week-old male C57BL/6J mice were fed either 2% parsley or basal diet and drank normal-drinking-water for 1 week after which colitis was induced by administering 1.5% (w/v) DSS-drinking-water for 9 days. Parsley supplementation significantly improved colon shortening and increased disease activity index. Colonic transcriptome revealed down-regulation of inflammatory cytokines, hepatic transcriptome and metabolome revealed up-regulation of fatty-acid synthesis genes, thereby improving body weight loss. Down-regulated cancer markers were observed in the hepatic transcriptome and proteome. Plasma metabolite analysis indicated shifts in the citric and urea cycle, implicating improved impaired glycolysis and oxidative stress. Parsley's role in preventing against IBD was highlighted by this pioneering multi-integrated-omics which is expected to be useful and informative, and shape the future of nutraceutical and functional food research. (C) 2014 The Authors. Published by Elsevier