Application of microCT in the study of the anatomy of small marine molluscs
Application of microCT in the study of the anatomy of small marine molluscs
复制标题
microCT在小型海洋软体动物解剖学研究中的应用
DOI：
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
V. Urgorri
中科院分区：
文献类型：
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作者：
M. Candás;G. DÍAZ;M. Abad;L. Barrio;X. Cunha;L. Pedrouzo;M. P. Señarís;Ó.;García;V. Urgorri
目的利用不同的技术手段对海洋生物进行外部解剖学研究（双目体视显微镜活体观察、光学显微镜观察、扫描电镜观察）。在扫描电镜的情况下，样品必须在扫描前进行预先处理。这些动物的内部解剖学研究是通过解剖或经典组织学技术进行的，这是一个复杂的过程，导致样品被破坏（在解剖的情况下）或切割成数百个薄切片，在光学显微镜下进行研究（在组织学的情况下）。当我们只有很少或只有一个物种的标本可供研究时，这就带来了一个问题。微计算机断层扫描是一种非侵入性技术，其允许在不破坏标本的情况下获得动物解剖结构的2D和3D信息（Hörnschemeyer等人，2002年;戈尔丁和琼斯，2007年）。这一特点使它成为一个很好的替代经典的方法时，研究类型的材料博物馆或独特的标本，是保持完整。一些作者（Golding & Jones，2007; Golding等人，2009; Metscher，2009; Faulwetter等人，2013 a，B; Candás等人，2014年）开展了关于在一些海洋生物中使用显微断层摄影技术的研究，并取得了良好的结果。通过这项工作，我们的目标是了解使用microCT在几组小型海洋软体动物的解剖学研究的有效性。
Aims Different techniques are used for the study of the external anatomy of marine organisms (in vivo observation under binocular stereo microscope, optical microscopy, SEM). In the case of SEM, samples must undergo a previous treatment before being scanned. The study of the internal anatomy of these animals is carried out through techniques of dissection or classical histology, which are complex processes resulting in the sample being destroyed (in the case of dissection) or cut in hundreds of thin sections which are studied under optical microscope (in the case of histology). This poses a problem when we have few or just one specimen of the species available for study. Micro-computed tomography is a non-invasive technique which allows to get information both in 2D and 3D of the anatomy of the animal without destroying the specimen (Hörnschemeyer et al., 2002; Golding & Jones, 2007). This characteristic makes it a good alternative to the classical methods when studying type material of museums or unique specimens which are to be kept intact. Some authors (Golding & Jones, 2007; Golding et al., 2009; Metscher, 2009; Faulwetter et al., 2013a,b; Candás et al., 2014) have carried out studies on the use of microtomography techniques in some marine organisms and obtained good results. With this work we aim to get to know the effectiveness of the use of microCT in the study of the anatomy of several groups of small-sized marine Mollusca.