An Altered DNA Methylation Status in the Human Umbilical Cord Is Correlated with Maternal Exposure to Polychlorinated Biphenyls
An Altered DNA Methylation Status in the Human Umbilical Cord Is Correlated with Maternal Exposure to Polychlorinated Biphenyls
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DOI：
10.3390/ijerph16152786
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发表时间：
2019-08-01
影响因子：
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通讯作者：
Mori, Chisato
中科院分区：
文献类型：
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作者：
Eguchi, Akifumi;Nishizawa-Jotaki, Shino;Mori, Chisato
母体暴露于多氯联苯（PCBs）导致胎儿发育异常，可能是由于表观遗传改变。然而，脐带血清中PCB水平与脐带组织中胎儿DNA甲基化状态之间的关系尚不清楚。本研究的目的是确定在脐带组织中的DNA甲基化的变化可能与PCB水平的脐带血清从出生队列在日本千叶（男性新生儿= 32，女性新生儿= 43）。甲基化阵列分析确定了雌性新生儿的5个位点（cg09878117、cg06154002、cg06289566、cg12838902、cg01083397）和雄性新生儿的1个位点（cg13368805），表明甲基化程度发生了变化。焦磷酸测序分析证实了这一结果，表明女性新生儿脐带组织中的cg06154002（tudor domain containing 9：TDRD9）与脐带血清中的总PCB水平显著相关。这些结果表明，暴露于多氯联苯可能会改变TDRD9甲基化水平，虽然这一假设需要进一步验证，从女性新生儿获得的数据。然而，由于目前的队列是小的，需要更大的队列进一步研究，以获得更多的数据，多氯联苯接触的影响，并确定相应的生物标志物。
Maternal exposure to polychlorinated biphenyls (PCBs) results in abnormal fetal development, possibly because of epigenetic alterations. However, the association between PCB levels in cord serum with fetal DNA methylation status in cord tissue is unclear. This study aims to identify alterations in DNA methylation in cord tissue potentially associated with PCB levels in cord serum from a birth cohort in Chiba, Japan (male neonates = 32, female neonates = 43). Methylation array analysis identified five sites for female neonates (cg09878117, cg06154002, cg06289566, cg12838902, cg01083397) and one site for male neonates (cg13368805) that demonstrated a change in the methylation degree. This result was validated by pyrosequencing analysis, showing that cg06154002 (tudor domain containing 9: TDRD9) in cord tissue from female neonates is significantly correlated with total PCB levels in cord serum. These results indicate that exposure to PCBs may alter TDRD9 methylation levels, although this hypothesis requires further validation using data obtained from female neonates. However, since the present cohort is small, further studies with larger cohorts are required to obtain more data on the effects of PCB exposure and to identify corresponding biomarkers.