Polyallamine-supported pseudo-stationary phases for electrokinetic chromatography Effect of alkyl chain length of the pseudo-stationary phase and methanol content of aqueous buffer on the separation of hydrophobic compounds
Polyallamine-supported pseudo-stationary phases for electrokinetic chromatography Effect of alkyl chain length of the pseudo-stationary phase and methanol content of aqueous buffer on the separation of hydrophobic compounds
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DOI：
10.1016/s0021-9673(97)00638-9
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发表时间：
1997-09
影响因子：
4.1
通讯作者：
N. Tanaka;K. Nakagawa;H. Iwasaki;K. Hosoya;K. Kimata;T. Araki;D. Patterson
中科院分区：
文献类型：
--
作者：
N. Tanaka;K. Nakagawa;H. Iwasaki;K. Hosoya;K. Kimata;T. Araki;D. Patterson
以市售聚烯丙胺（PAA）为原料，制备了烷基（c8 - c16）和羧酸基的高分子假固定相。PAA首先与烷基溴烷基化，然后与丙烯酸甲酯反应，随后水解酯官能团。paa负载的伪固定相，特别是具有长烷基链的假固定相，在广泛的缓冲液-甲醇混合物中为烷基苯基酮和多核芳烃提供了高效率。疏水化合物的迁移次数。在甲醇含量较低时，受载体迁移时间（tc.）的限制，在甲醇含量为40-60%时表现出较大的增加，而在甲醇含量较高时，由于k'值的减少而出现较大的t - C值。当甲醇含量较高，烷基较长时，准固定相的迁移时间窗扩大。甲醇加入对不同烷基PAA衍生物行为影响的差异可归因于聚合物伪固定相的结构变化，导致相对于电渗透流动的电泳迁移率更高，从而产生较大的tc值。聚丙烯酸负载的伪固定相制备简单，效率高，将使聚合物伪固定相的电动色谱法成为一种很有前途的工具，可用于分离简单水-甲醇混合物中的多种化合物，包括疏水多核芳烃。
Polymeric pseudo-stationary phases having alkyl (c 8-c 16) and carboxylate groups were prepared from commercially available polyallylamine (PAA). PAA was first alkylated with an alkyl bromide, then reacted with methyl acrylate, followed by the hydrolysis of the ester functionality. The PAA-supported pseudo-stationary phases, especially those with long alkyl chains, provided high efficiencies for alkyl phenyl ketones and polynuclear aromatic hydrocarbons in a wide range of buffer-methanol mixtures. Migration times of the hydrophobic compounds. limited by the migration time of the carrier (tc.) at low methanol content, showed a large increase at 40-60% methanol, and decreased due to the decrease in k'values at higher methanol content where large t C values were observed. The expansion of the migration time window occurred at a higher methanol content with the pseudo-stationary phase with the longer alkyl groups. Difference in the effect of methanol addition on the behavior of PAA derivatives with different alkyl groups can be attributed to the structural change of the polymeric pseudo-stationary phase resulting in the greater electrophoretic mobility relative to electroosmotic flow, and in turn large tc values. Simple preparation of the PAA-supported pseudo-stationary phase and the high efficiency will make electrokinetic chromatography using polymeric pseudo-stationary phases a promising tool for the separation of a wide range of compounds including very hydrophobic polynuclear aromatic hydrocarbons in simple water-methanol mixtures.