Microtubule Detachment in Gliding Motility Assays Limits the Performance of Kinesin-Driven Molecular Shuttles
Microtubule Detachment in Gliding Motility Assays Limits the Performance of Kinesin-Driven Molecular Shuttles
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DOI：
10.1021/acs.langmuir.0c01002
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发表时间：
2020-07-14
期刊：
影响因子：
3.9
通讯作者：
Hess, Henry
中科院分区：
文献类型：
--
作者：
Kazeruni, Neda M. Bassir;Rodriguez, Juan B., III;Hess, Henry
由表面粘附的马达蛋白推动的整合细胞骨架细丝的复杂活性纳米系统的创建通常依赖于细丝滑行长达一米的距离的能力。虽然理论上的考虑支持这种能力，我们表明，微管脱离（无论是自发的或触发的微管交叉事件）是一个不可忽视的现象，一直被忽视到现在。在1 mM ATP下，对于500 μ m（-2）的功能性驱动蛋白-1密度，测量自发脱离前的平均滑动距离为30 +/-10 mm，对于100 μ m（-2）的功能性驱动蛋白-1密度，测量自发脱离前的平均滑动距离为9 +/-4 mm。甚至微管长于3 μ m的脱落，表明自发脱离是不是由于随机缺乏电机或随机释放，由于有限的运行长度。
The creation of complex active nanosystems integrating cytoskeletal filaments propelled by surface-adhered motor proteins often relies on the filaments' ability to glide over up to meter-long distances. While theoretical considerations support this ability, we show that microtubule detachment (either spontaneous or triggered by a microtubule crossing event) is a non-negligible phenomenon that has been overlooked until now. The average gliding distance before spontaneous detachment was measured to be 30 +/- 10 mm for a functional kinesin-1 density of 500 mu m(-2) and 9 +/- 4 mm for a functional kinesin-1 density of 100 mu m(-2) at 1 mM ATP. Even microtubules longer than 3 mu m detached, suggesting that spontaneous detachment is not caused by the stochastic absence of motors or their stochastic release due to a limited run length.