ESTs analysis of putative genes engaged in Polyporus umbellatus sclerotial development.
ESTs analysis of putative genes engaged in Polyporus umbellatus sclerotial development.
复制标题
猪苓菌核发育相关基因的 EST 分析
DOI：
10.3390/ijms150915951
复制
发表时间：
2014-09-10
影响因子：
5.6
通讯作者：
Guo S
中科院分区：
文献类型：
--
作者：
Song C;Liu M;Xing Y;Guo S
Polyporus umbellatus is one of the most widely used and precious medicinal fungi and the underground sclerotia are known to be with great medicinal value. However, the molecular mechanisms involved in sclerotial development are poorly understood. In the present study, we constructed a forward suppression subtractive hybridization (SSH) cDNA library of Polyporus umbellatus to identify genes expressing differently between mycelium and sclerotia. In this library, a total of 1202 clones were sequenced, assembled into 222 contigs and 524 singletons which were further searched against the NCBI nonredundant (NR) protein database (E-value cutoff, 10−5). Based on sequence similarity with known proteins, 378 sequences between mycelium and sclerotial were identified and classified into different functional categories through Gene Ontology (GO), Clusters of orthologous Groups of proteins (COGs). We have finally identified a majority of differentially expressed genes (constituting 5.6% of the present library) between the two different periods. An expression level of 32 selected expressed sequence tags (ESTs) generated from the above SSH cDNA library was studied through RT-PCR. This study provides the first global overview of genes putatively involved in Polyporus umbellatus sclerotial development and provides a preliminary basis for further functional research in terms of regulated gene expression in sclerotial production.
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影响因子：
--
作者：
Georgiou, CD
通讯作者：
Georgiou, CD
DOI：
10.1016/j.jcice.2008.01.003
发表时间：
2008-07-01
期刊：
JOURNAL OF THE CHINESE INSTITUTE OF CHEMICAL ENGINEERS
影响因子：
--
作者：
Huang, Hung-Chang;Liu, Yung-Chuan
通讯作者：
Liu, Yung-Chuan
Ca2 通道信号传导参与猪苓菌核形成
影响因子：
5.5
作者：
Liu, Ying-Ying;Guo, Shun-Xing
通讯作者：
Guo, Shun-Xing
影响因子：
1.9
作者：
Kennedy, MA;Johnson, TA;Bard, M
通讯作者：
Bard, M
影响因子：
3.2
作者：
Harel, A;Gorovits, R;Yarden, O
通讯作者：
Yarden, O