Gene expression during liver regeneration.
Gene expression during liver regeneration.
复制标题
肝再生过程中的基因表达。
DOI：
10.1016/0022-2836(84)90305-x
复制
发表时间：
1984
影响因子：
5.6
通讯作者：
DarnellJr,JE
中科院分区：
文献类型：
--
作者：
Friedman,JM;Chung,EY;DarnellJr,JE
在肝再生过程中，检测了一组信使RNA的浓度，其中一些只(或主要)在肝脏表达，另一些在所有组织中表达。大多数组织特异性的mRNAs在浓度或转录速率上没有太大变化，但肌动蛋白和微管蛋白等mRNAs的转录增加了10倍，而转录没有相应的增加。然而，“急性期”蛋白质如血清淀粉样蛋白A和金属硫氨酸的mRNAs确实显著增加，而且很容易检测到转录增加。除了这些发现，核糖体、核糖体蛋白的前rRNA和mRNA的合成所需的RNA成分的合成速度没有增加。因此，分化的肝细胞在再生所需的两次左右的复制过程中继续发挥分化细胞的功能，许多增加细胞质量所必需的成分可能是通过增加转录RNA的保存和利用来提供的。
The concentration of a group of messenger RNAs, some of which are expressed only (or mainly) in the liver and others of which are expressed in all tissues, was examined during liver regeneration. Most of the tissue-specific mRNAs did not change greatly in concentration or in transcription rate, but mRNAs such as actin and tubulin increased by as much as tenfold without an equivalent transcriptional increase. However, the mRNAs for “acute phase” proteins such as serum amyloid A and metallothionine did increase dramatically and increased transcription was easily detected. In addition to these findings, there was no increase in the rate of synthesis of the RNA constituents necessary to make ribosomes, pre-rRNA and mRNA for ribosomal proteins. Thus, the differentiated hepatocyte continues to function as a differentiated cell during the two or so replications necessary for regeneration, and many of the constituents necessary to increase cell mass may be supplied by increased preservation and utilization of transcribed RNAs.