Detection and serotyping of Streptococcus pneumoniae from nasopharyngeal samples by PCR-based multiplex assay
Detection and serotyping of Streptococcus pneumoniae from nasopharyngeal samples by PCR-based multiplex assay
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DOI：
10.1128/jcm.43.12.6152-6154.2005
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发表时间：
2005-12-01
影响因子：
9.4
通讯作者：
Castañeda, E
中科院分区：
文献类型：
--
作者：
Moreno, J;Hernández, E;Castañeda, E
我们开发了一种基于多重 PCR 的方法，用于保存在鸡蛋巯基乙酸盐抗生素和脱脂牛奶-胰蛋白胨-葡萄糖-甘油培养基中的鼻咽样本，以识别和血清分型引起儿童侵袭性疾病的肺炎链球菌最重要的血清型。该技术可用于研究肺炎球菌定植的流行病学和结合疫苗的效果。
We developed a multiplex PCR-based methodology for nasopharyngeal samples maintained in egg thioglycolate antibiotic and skim milk-tryptone-glucose-glycerol media to identify and serotype the most important serotypes of Streptococcus pneumoniae that cause invasive disease in children. This technique can be used to study the epidemiology of pneumococcal colonization and the effect of conjugate vaccines.