H+-INDUCED RELEASE OF CONTENTS OF PHOSPHATIDYLCHOLINE VESICLES BEARING SURFACE-BOUND POLY-ELECTROLYTE CHAINS
H+-INDUCED RELEASE OF CONTENTS OF PHOSPHATIDYLCHOLINE VESICLES BEARING SURFACE-BOUND POLY-ELECTROLYTE CHAINS
复制标题
DOI：
10.1021/ja00230a028
复制
发表时间：
1988-10-26
影响因子：
15
通讯作者：
TIRRELL, DA
中科院分区：
文献类型：
--
作者：
MAEDA, M;KUMANO, A;TIRRELL, DA
通过将合成聚电解质[聚（2-乙基丙烯酸）]固定在磷脂酰胆碱双层表面上构建半合成囊泡膜。通过将聚合物结合的硫醇官能团Michael加成至以二肉豆蔻酰-N-[[4-（马来酰亚胺甲基）环己基]羰基]磷脂酰乙醇胺的形式并入脂质膜中的反应性马来酰亚胺柄来实现固定化。以这种方式制备的半合成膜对氢离子浓度敏感，并且在pH值变化小的情况下渗透性变化大。囊泡内容物的快速定量释放可以通过在生理pH值范围内的温和酸化来实现。
A semisynthetic vesicular membrane was constructed by immobilization of a synthetic polyelectrolyte [poly(2-ethylacrylic acid)] on the surface of a phosphatidylcholine bilayer. Immobilization was accomplished via Michael addition of polymer-bound thiol functions to a reactive maleimide handle incorporated into the lipid membrane in the form of dimyristoyl-N-[[4-(maleimidomethyl)cyclohexyl]carbonyl]phosphatidylethanolamine. Semisynthetic membranes prepared in this way are sensitive to hydrogen ion concentration and are subject to large variations in permeability with small changes in pH. Rapid and quantitative release of vesicle contents can be achieved by mild acidification within the physiological pH range.