Sex differences in the renal transforming growth factor-β1 system after puberty
Sex differences in the renal transforming growth factor-β1 system after puberty
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DOI：
10.1007/s004670000502
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发表时间：
2001-01-01
影响因子：
3
通讯作者：
Langer, WJ
中科院分区：
文献类型：
--
作者：
Lane, PH;Snelling, DM;Langer, WJ
转化生长因子-β1(TGF-β1)与多种进展性肾脏疾病密切相关。本研究检测了青春期和成年期早期的这种生长因子。慕尼黑-Wistar混合性别大鼠肾脏取自出生至6个月龄的特定天数。对转化生长因子-β1系统进行了调查，然后对6周龄和16周龄的两性的皮质和髓质进行了第二次实验。逆转录-聚合酶链式反应检测转化生长因子-β1和转化生长因子-β诱导基因H3(β-IG-HS)。从组织中分离转化生长因子-β1的活性蛋白和总蛋白水平。在年龄较大的动物中，转化生长因子-β1的活性水平略低于年轻动物，没有性别差异。β-IG-H3水平相似。在16周时，雌性的总生长因子水平大约是雄性的三倍，而成年雄性似乎更有效地激活了生长因子。这些发现表明，在雄性大鼠青春期后，转化生长因子-β1的激活变得更有效，而雌性大鼠的激活效率似乎降低了，而总生长因子的增加弥补了这一点。这些差异可能有助于解释一些进行性肾病的青春期恶化和性二型性。
Transforming growth factor-beta1 (TGF-beta1) has been implicated in many progressive kidney diseases. The present study examines this growth factor during the pubertal and early adult periods. Mixed-sex Munich-Wistar rat kidneys were obtained on selected days of life from birth through 6 months of age. A survey of the TGF-beta1 system was performed, and then a second experiment focused on cortex and medulla from both sexes at 6 weeks and 16 weeks of age. Reverse transcription polymerase chain reaction for TGF-beta1 and TGF-beta inducible gene H3 (beta IG-HS) was performed. Active and total levels of protein for TGF-beta1 were isolated from tissue. Active levels of TGF-beta1 were somewhat lower in older than in younger animals, without sex differences. beta IG-H3 levels were similar. At 16 weeks females had levels of total growth factor approximately threefold greater than males, while adult males appeared to activate the growth factor much more efficiently. These findings suggest that activation of TGF-beta1 becomes more efficient following puberty in the male rat, while females appear to have reduced activation efficiency compensated by increased total growth factor. These differences may help explain the deterioration at puberty and sexual dimorphism noted with some progressive nephropathies.