Effect of ethanol and acetaldehyde on intracellular protease activities in human liver, brain and muscle tissues in vitro.
Effect of ethanol and acetaldehyde on intracellular protease activities in human liver, brain and muscle tissues in vitro.
复制标题
乙醇和乙醛对体外人肝、脑和肌肉组织细胞内蛋白酶活性的影响。
DOI：
10.1016/s0009-8981(98)00211-3
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
V. Preedy
中科院分区：
文献类型：
--
作者：
David Mantle;Gavin Falkous;T. Peters;V. Preedy
在体外测定了乙醇和乙醛对人脑、肝脏和骨骼肌组织中一系列胞浆和溶酶体蛋白水解酶活性的影响。当乙醇(10%，v/v；1.7m o l/L)或乙醛(1%，v/v；0.17m o l/L)浓度足够高时，对所有组织中的大多数蛋白水解酶都有相当程度的抑制。然而，我们得出的结论是，乙醇或乙醛不可能在体内直接抑制蛋白酶，因为这些药物的浓度很可能与体内的蛋白分解代谢有关，而且这些药物对细胞内蛋白质分解代谢的任何影响都必须通过更微妙的生物化学机制发生。
The effect of ethanol and acetaldehyde on the activity of a range of intracellular cytoplasmic and lysosomal proteolytic enzymes has been determined in human brain, liver and skeletal muscle tissues in vitro. There was a substantial degree of inhibition for most protease types in all tissues if sufficiently high concentrations of ethanol (10%, v/v; 1.7 mol/L) or acetaldehyde (1%, v/v; 0.17 mol/L) were used in the assay media. However, it was concluded that direct inhibition of proteases in vivo by ethanol or acetaldehyde is improbable, at the concentrations of these agents likely to pertain in vivo, and that any effect of these agents on intracellular protein catabolism must occur via a more subtle biochemical mechanism.