Effects of exercise training on gingival oxidative stress in obese rats
Effects of exercise training on gingival oxidative stress in obese rats
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DOI：
10.1016/j.archoralbio.2011.01.008
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发表时间：
2011-08-01
影响因子：
3
通讯作者：
Morita, Manabu
中科院分区：
文献类型：
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作者：
Azuma, Tetsuji;Tomofuji, Takaaki;Morita, Manabu
目的：探讨运动训练对高脂饮食肥胖大鼠血清活性氧(ROS)水平和牙龈氧化应激的影响。设计：将肥胖大鼠分为3组(n=14)：1对照组(常规饲料喂养)和2个实验组(高脂饲料+运动训练+和不运动训练+跑台：5天/周)。分别于4周或8周处死大鼠。测定血清反应性氧化代谢产物(ROM)水平，作为循环ROS的指标。结果：高脂饲料喂养4周后，肥胖大鼠血清Carratelli Units(Carratelli Units)[Carratelli Units(Carratelli Units，Carratelli Units)](均值+/-SD；413+/-)明显高于对照组(P=0.023)。与对照组(8-OHdG为0.55+/-0.13，GSH/GSSG为23.6+/-5.8)相比，8周时8-OHdG水平(ng/mg mtDNA)(0.97+/-0.18)升高(p<；0.05)，GSH/GSSG比值(17.0+/-3.1)降低(p<；0.05)。此外，高脂饮食加运动训练的肥胖大鼠血清ROM值较低(623+/-103)(P<0.05)。
Objective: The purpose of the present study was to investigate the effects of exercise training on serum reactive oxygen species (ROS) level and gingival oxidative stress in obese rats fed a high-fat diet.Design: Rats were divided into three groups (n = 14/group): one control group (fed a regular diet) and two experimental groups (fed a high-fat diet with and without exercise training [treadmill: 5 days/week]). The rats were sacrificed at 4 or 8 weeks. The level of serum reactive oxidative metabolites (ROM) was measured as an indicator of circulating ROS. The level of 8-hydroxydeoxyguanosine (8-OHdG) and reduced-form glutathione (GSH)/oxidised-form glutathione (GSSG) ratio were determined to evaluate gingival oxidative stress.Results: The obese rats fed a high-fat diet without exercise training showed higher serum ROM levels [Carratelli Units (CARR U)] (mean +/- SD; 413 +/- 64) than the control (333 +/- 12) at 4 weeks (p = 0.023). Such a condition resulted in higher 8-OHdG levels (ng/mg mtDNA) (0.97 +/- 0.18) (p < 0.05) and a lower GSH/GSSG ratio (17.0 +/- 3.1) (p < 0.05) in gingival tissues, compared to the control (0.55 +/- 0.13 for 8-OHdG and 23.6 +/- 5.8 for GSH/GSSG ratio) at 8 weeks. In addition, the obese rats fed a high-fat diet with exercise training showed lower serum ROM (623 +/- 103) (p