Effect of carbon nanotubes on cellular functions in vitro
Effect of carbon nanotubes on cellular functions in vitro
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DOI：
10.1002/jbm.a.32203
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发表时间：
2009-10-01
影响因子：
4.9
通讯作者：
Watari, Fumio
中科院分区：
文献类型：
--
作者：
Li, Xiaoming;Gao, Hong;Watari, Fumio
碳纳米管（CNT）已被证明会影响细胞行为。但是，碳纳米管如何以及为什么影响附着细胞的潜在分化还没有很大程度上知道。在这项研究中，多壁碳纳米管（MWNTs）和石墨（GP）压制成压块。与GP相比，CNT显示出更高的吸附蛋白质的能力。体外培养小鼠成肌细胞（C2 C12），比较细胞对两种压块的反应。同时，我们使用培养板上的细胞培养物作为对照。在常规培养过程中，发现细胞在MWNTs上的附着、增殖和分化明显更好。为了证实碳纳米管上吸附的大量蛋白质对此至关重要的假设，我们在细胞培养前，在含有50%胎牛血清（FBS）的培养基中使压块吸附更多的蛋白质。随着蛋白质的预先吸附，MWNTs的总蛋白/DNA和碱性磷酸酶（ALP）/DNA的增量分别为GP和对照的增量的11倍和18倍。因此，碳纳米管可能通过吸附更多的蛋白质来诱导细胞功能，这表明碳纳米管可能是组织工程支架材料的候选者。(C)2008 Wiley Periodicals，Inc. J Biomed Mater Res 91A：132-139，2009
Carbon nanotubes (CNTs) have been shown to affect cell behavior. But how and why the CNTs affect potential differentiation of the attached cells has not been largely known. In this study, multiwalled carbon nanotubes (MWNTs) and graphite (GP) were pressed as compacts. Higher ability of CNTs to adsorb proteins, compared with GP, was shown. Myoblastic mouse cells (C2C12) were cultured and the cell responses to the two kinds of compacts were compared in vitro. Meanwhile, we used cell culture on the culture plate as a control. During the conventional culture, significantly better cell attachment, proliferation, and differentiation of cells on the MWNTs were found. To confirm the hypothesis that the larger amount of protein adsorbed on the CNTs was crucial for this, we made the compacts adsorb more proteins in culture medium with 50% fetal bovine serum (FBS) before cell culture. With the adsorption of the proteins in advance, the increments of the total-protein/DNA and alkaline phosphatase (ALP)/DNA for the MWNTs was respectively as about 11 times and 18 times as the increments of those for GP and the control at both day 4 and day 7. Therefore, the CNTs might induce cellular functions by adsorbing more proteins, which indicated that the CNTs might be a candidate for scaffold material for tissue engineering. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res 91A: 132-139, 2009