Early transcriptomic events in microdissected Arabidopsis nematode-induced giant cells
Early transcriptomic events in microdissected Arabidopsis nematode-induced giant cells
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DOI：
10.1111/j.1365-313x.2009.04098.x
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发表时间：
2010-02-01
期刊：
影响因子：
7.2
通讯作者：
Escobar, Carolina
中科院分区：
文献类型：
--
作者：
Barcala, Marta;Garcia, Alejandra;Escobar, Carolina
根结线虫通过包括广泛的转录变化在内的特征不明确的机制，分化出根中高度特化的饲养细胞（巨细胞，GC）。虽然全球转录组分析使用了虫瘿，这是复杂的根结构，包括GC和周围组织，没有全球基因表达变化的GC已被描述。我们报告的差异转录组的GC与根维管细胞，诱导拟南芥根结线虫在其发展的早期阶段，感染后3天（d.p.i.）。激光显微切割用于捕获GC和根维管细胞用于微阵列分析，其通过qPCR和启动子-GUS融合研究进行验证。结果显示，通过3d.p.i.，GC表现出主要的基因抑制。虽然一些基因在虫瘿和GC中表现出相似的调控，但大多数基因具有不同的表达模式，证实了GCs在虫瘿中的分子特异性。GC中的大多数差异调节基因先前没有指定的功能。与其他转录组分析的比较揭示了GC和细胞悬浮液分化成木质部细胞之间的相似性。这表明GC和发育中的血管细胞（代表推定的GC干细胞）之间存在分子联系。在3 d.p.i.也被发现类似于根癌农杆菌，一个专门的根生物营养诱导冠瘿。
Root-knot nematodes differentiate highly specialized feeding cells in roots (giant cells, GCs), through poorly characterized mechanisms that include extensive transcriptional changes. While global transcriptome analyses have used galls, which are complex root structures that include GCs and surrounding tissues, no global gene expression changes specific to GCs have been described. We report on the differential transcriptome of GCs versus root vascular cells, induced in Arabidopsis by Meloidogyne javanica at a very early stage of their development, 3 days after infection (d.p.i.). Laser microdissection was used to capture GCs and root vascular cells for microarray analysis, which was validated through qPCR and by a promoter-GUS fusion study. Results show that by 3 d.p.i., GCs exhibit major gene repression. Although some genes showed similar regulation in both galls and GCs, the majority had different expression patterns, confirming the molecular distinctiveness of the GCs within the gall. Most of the differentially regulated genes in GCs have no previously assigned function. Comparisons with other transcriptome analyses revealed similarities between GCs and cell suspensions differentiating into xylem cells. This suggests a molecular link between GCs and developing vascular cells, which represent putative GC stem cells. Gene expression in GCs at 3 d.p.i. was also found to be similar to crown galls induced by Agrobacterium tumefaciens, a specialized root biotroph.