Involvement of Arabidopsis Acyl Carrier Protein 1 in PAMP-Triggered Immunity
Involvement of Arabidopsis Acyl Carrier Protein 1 in PAMP-Triggered Immunity
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DOI：
10.1094/mpmi-02-22-0049-r
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发表时间：
2022-08-01
影响因子：
3.5
通讯作者：
Xia，Ye
中科院分区：
文献类型：
--
作者：
Zhao，Zhenzhen;Fan，Jiangbo;Xia，Ye
植物脂肪酸（FA）和脂质对于储存能量至关重要，并作为细胞膜的结构成分和植物生长和胁迫反应的信号分子。酰基载体蛋白（Acyl carrier protein，ACP）是一种小分子酸性蛋白，在脂肪酸的延伸过程中与脂肪酰基中间体共价结合。拟南芥ACP家族有8个成员。通过反向遗传，分子和生物化学方法，我们已经发现，ACP 1定位于叶绿体和限制的模式触发免疫（PTI）对细菌病原体假单胞菌。番茄的幅度。Mutantacp 1植物的亚麻酸（18：3）水平降低，这是生物合成植物激素茉莉酸（JA）的主要前体，JA的丰度相应减少。与已知的JA和水杨酸（SA）之间的拮抗关系相一致，acp 1突变体植物也积累了更高水平的SA，并在JA和SA调节的转录输出中显示相应的变化。此外，JA甲酯和亚麻酸处理还明显增强了对磷的抗性下降。与野生型植株相比，番茄epv.tomatoinacp1突变体对番茄的抗性更强。ACP 1阻止这种激素失衡的能力可能是其对植物防御中PTI的负面影响的基础。因此，ACP 1将FA代谢与胁迫激素稳态联系起来，从而负面地参与PTI inflammopsis植物防御。版权所有© 2022作者。这是一篇开放获取的文章，在CC BY-NC-ND 4.0国际许可下分发。
Plant fatty acids (FAs) and lipids are essential in storing energy and act as structural components for cell membranes and signaling molecules for plant growth and stress responses. Acyl carrier proteins (ACPs) are small acidic proteins that covalently bind the fatty acyl intermediates during the elongation of FAs. TheArabidopsis thalianaACP family has eight members. Through reverse genetic, molecular, and biochemical approaches, we have discovered that ACP1 localizes to the chloroplast and limits the magnitude of pattern-triggered immunity (PTI) against the bacterial pathogenPseudomonas syringaepv.tomato. Mutantacp1plants have reduced levels of linolenic acid (18:3), which is the primary precursor for biosynthesis of the phytohormone jasmonic acid (JA), and a corresponding decrease in the abundance of JA. Consistent with the known antagonistic relationship between JA and salicylic acid (SA),acp1mutant plants also accumulate a higher level of SA and display corresponding shifts in JA- and SA-regulated transcriptional outputs. Moreover, methyl JA and linolenic acid treatments cause an apparently enhanced decrease of resistance againstP. syringaepv.tomatoinacp1mutants than that in WT plants. The ability of ACP1 to prevent this hormone imbalance likely underlies its negative impact on PTI in plant defense. Thus, ACP1 links FA metabolism to stress hormone homeostasis to be negatively involved in PTI inArabidopsisplant defense.Copyright © 2022 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.