Microbial degradation of dissolved proteins in seawater1
Microbial degradation of dissolved proteins in seawater1
复制标题
海水中溶解蛋白质的微生物降解1
DOI：
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发表时间：
1983
期刊：
影响因子：
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通讯作者：
F. Azam
中科院分区：
文献类型：
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作者：
J. Hollibaugh;F. Azam
利用放射性标记蛋白质的实验方案，研究了天然海洋浮游生物群落中溶解蛋白质降解的速率和机制。野外观察和实验室实验的结果表明，蛋白质降解的颗粒结合，热不稳定的系统，可能是细菌相关的酶，与一个表观半饱和常数约。二十五... Mu.. g牛血清白蛋白（BSA）/L。凝胶渗透色谱表明，链长介于BSA和最终降解产物之间的肽（MW<700）不会在培养基中积累。竞争实验表明，该系统是相对非特异性的。周转率的蛋白池在南加州湾收集的样品中的周转率的L-亮氨酸池的周转率是相同的数量级，并与初级生产力，叶绿素a浓度，细菌丰度和生物量，和L-亮氨酸周转率。这些数据表明，来自蛋白质的氨基酸被优先利用，并且不与本体相中的氨基酸完全混合。
An experimental protocol using radiolabeled proteins was developed to investigate the rates and mechanisms whereby dissolved proteins are degraded in natural marine plankton communities. The results of field observations and laboratory experiments indicate that proteins are degraded by a particle-bound, thermolabile system, presumably bacteria-associated enzymes, with an apparent half-saturation constant of ca. 25 ..mu..g bovine serum albumin (BSA) per liter. Gel permeation chromatography indicated that peptides of chain length intermediate between BSA and the final products of degradation (MW<700) do not accumulate in the medium. Competition experiments indicate that the system is relatively nonspecific. Turnover rates for the protein pool in samples collected in the Southern California Bight were of the same order of magnitude as the turnover rate of the L-leucine pool and were correlated with primary productivity, chlorophyll a concentrations, bacterial abundance and biomass, and L-leucine turnover rate. These data suggest that amino acids derived from proteins are utilized preferentially and do not completely mix with the amino acids in the bulk phase.