Endogenous inhibitors of lysosomal proteinases.
Endogenous inhibitors of lysosomal proteinases.
复制标题
溶酶体蛋白酶的内源性抑制剂。
DOI：
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发表时间：
1983
影响因子：
11.1
通讯作者：
B. Horecker
中科院分区：
文献类型：
--
作者：
S. Pontremoli;E. Melloni;F. Salamino;B. Sparatore;M. Michetti;B. Horecker
兔肝的胞质和溶酶体区室中存在三种溶酶体蛋白酶的特异性抑制剂。胞浆抑制剂通过 DEAE-Trisacryl 和 Sephadex G-75 色谱纯化，分别对组织蛋白酶 M、组织蛋白酶 B 和 L 以及果糖 1,6-二磷酸酶转换酶 (CE) 表现出特异性，并分别命名为 IM、IB/L 和 ICE。具有相似特异性的抑制剂已从溶酶体内隔室中分离出来。其中两种抑制剂 IM 和 ICE 也存在于溶酶体膜中。溶酶体分布与相应蛋白酶的分布平行。对于IB/L的两种形式，抑制剂是分子量为5,000-10,000的多肽，对于IM，抑制剂是分子量12,500，对于ICE种类，抑制剂是分子量10,000-40,000。
Specific inhibitors of three lysosomal proteinases are present in the cytosolic and lysosomal compartments of rabbit liver. The cytosolic inhibitors, purified by chromatography on DEAE-Trisacryl and Sephadex G-75, show specificities toward cathepsin M, cathepsins B and L, and fructose 1,6-bisphosphatase converting enzyme (CE), respectively, and are designated IM, IB/L, and ICE. Inhibitors with similar specificities have been isolated from the intralysosomal compartment. Two of these inhibitors, IM and ICE, are also present in the lysosomal membranes. The lysosomal distribution parallels that of the respective proteinases. The inhibitors are polypeptides with molecular weights of 5,000-10,000 for the two forms of IB/L, 12,500 for IM, and 10,000-40,000 for the ICE species.