Immunoglobulin protein and gene transcripts in ovarian follicles throughout oogenesis in the teleost Dicentrarchus labrax
Immunoglobulin protein and gene transcripts in ovarian follicles throughout oogenesis in the teleost Dicentrarchus labrax
复制标题
DOI：
10.1007/s00441-003-0819-9
复制
发表时间：
2004-02-01
影响因子：
3.6
通讯作者：
Abelli, L
中科院分区：
文献类型：
--
作者：
Picchietti, S;Taddei, AR;Abelli, L
免疫球蛋白（IgM 样）从雌性转移到硬骨鱼胚胎已得到证实，但卵中摄取和储存的机制仍有待澄清。针对 Dicentrarchus labrax Ig 轻链的单克隆抗体 DLIg3 揭示了卵泡细胞和卵母细胞在 Ig 摄取中的积极作用。即使在卵黄发生前阶段，原始滤泡细胞也表现出 DLIg3 免疫反应性。早期卵母细胞（脂质囊泡阶段）的孔管、质膜和外皮层及其滤泡细胞进行了 DLIg3 染色。在蛋白卵黄颗粒卵母细胞中，外中皮质囊泡和近核卵黄颗粒内也检测到 DLIg3 染色。因此，Ig 在卵母细胞发育过程中显然是进行向心运输的。免疫电镜证实卵黄发生前和卵黄发生卵泡的鞘膜和颗粒细胞（以及插入的基底膜）内存在 Ig。因此，Ig 向卵子的转运显然也是通过卵泡细胞的转胞吞作用发生的。 Igs 位于微绒毛周围的孔道和卵黄卵母细胞外中皮层的囊泡中。使用针对鲈鱼 Ig 轻链恒定区设计的引物进行逆转录/聚合酶链反应，检测到水合卵母细胞中的 Ig mRNA，释放的卵中含量较少，孵化后第二天幼虫中没有信号。这些发现表明，卵母细胞中 Ig 基因转录和/或转录本的转移也可能发生显着水平。
Transfer of immunoglobulins (IgM-like) from the female to the teleost embryo has been demonstrated but mechanisms of uptake into and storage within the eggs remain to be clarified. The monoclonal antibody DLIg3 against Dicentrarchus labrax Ig light chain revealed an active role of both follicle cells and oocytes in the Ig uptake. The primordial follicular cells showed DLIg3 immunoreactivity even at a pre-vitellogenetic stage. Early vitellogenetic oocytes (lipid vesicle stages) had DLIg3 staining of pore canals, plasmalemma and outer cortex and of their follicular cells. In protein yolk granule oocytes, DLIg3 staining was also detected within vesicles of the outer-mid cortex and juxtanuclear yolk granules; therefore, a centripetal transport of Ig throughout oocyte development is apparently carried out. Immunoelectron microscopy confirmed the presence of Ig within thecal and granulosa cells (and in the interposed basement membrane) of pre-vitellogenic and vitellogenic follicles. Thus, the transport of Ig to the egg apparently occurs also by transcytosis across the follicle cells. Igs were localised in the pore canals surronding the microvilli and in vesicles of outer-mid cortex of vitellogenic oocytes. Reverse transcription/polymerase chain reaction with primers designed for the constant region of sea bass Ig light chain detected Ig mRNA in hydrated oocytes, a smaller content in released eggs and no signal in larvae at day two post-hatching. These findings show that a significant level of Ig gene transcription in the oocyte and/or a transfer of transcripts may also occur.