Mapping T and B lymphocyte epitopes of bovine herpesvirus-1 glycoprotein B
Mapping T and B lymphocyte epitopes of bovine herpesvirus-1 glycoprotein B
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DOI：
10.1099/0022-1317-80-10-2699
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发表时间：
1999-10-01
影响因子：
3.8
通讯作者：
Splitter, GA
中科院分区：
文献类型：
--
作者：
Gao, Y;Wang, C;Splitter, GA
糖蛋白B（gB）是牛疱疹病毒1型（BHV-1）的主要囊膜蛋白，重组gB的胞外结构域作为亚单位疫苗，可诱导中和抗体和T细胞应答，产生抗病毒攻击的保护作用。在此，分析了来自不同亲本动物的淋巴细胞对gB胞外结构域的T细胞增殖以进行免疫识别。从杆状病毒载体表达编码742个氨基酸的四个截短的重叠gB基因片段以鉴定抗原区域。一个免疫显性区域（氨基酸254-532）被来自不同亲本的免疫个体的T细胞识别。跨越该区域的系列合成肽定位T细胞（氨基酸319-340和415-436）和B细胞（氨基酸331-352、475-496和487-508）表位。gB表位的阐明表明，牛（BHV-1的天然宿主）的T细胞和抗体对这种包膜糖蛋白具有多样性和独特性的识别。
Glycoprotein B (gB) is a major envelope protein of bovine herpesvirus-1 (BHV-1), As a subunit vaccine, the extracellular domain of recombinant gB induces neutralizing antibody and T cell responses that engender protection against virus challenge. Here, lymphocytes from animals of different parentage were analysed for T cell proliferation to the gB extracellular domain for immune recognition. Four truncated overlapping gB gene segments encoding 742 amino acids were expressed from a baculovirus vector to identify antigenic regions. One immunodominant region (amino acids 254-532) was recognized by T cells from immune individuals of different parentage. Serial synthetic peptides spanning this region localized the T cell (amino acids 319-340 and 415-436) and B cell (amino acids 331-352, 475-496 and 487-508) epitopes. Elucidation of gB epitopes indicates the diverse and distinctive recognition by T cells and antibodies of this envelope glycoprotein by cattle, the natural host of BHV-1.