B-CELL AND IMMUNOGLOBULIN HETEROGENEITY IN CARP (CYPRINUS-CARPIO L) - AN IMMUNO(CYTO)CHEMICAL STUDY
B-CELL AND IMMUNOGLOBULIN HETEROGENEITY IN CARP (CYPRINUS-CARPIO L) - AN IMMUNO(CYTO)CHEMICAL STUDY
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DOI：
10.1016/0145-305x(94)00061-j
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发表时间：
1995-01-01
影响因子：
2.9
通讯作者：
ROMBOUT, JHWM
中科院分区：
文献类型：
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作者：
KOUMANSVANDIEPEN, JCE;EGBERTS, E;ROMBOUT, JHWM
B细胞和免疫球蛋白（IG）异质性在鲤鱼，鲤鱼L.，用两株抗鲤鱼血清IG的单克隆抗体（WC 14、WCI 12）进行检测。免疫化学结果显示，WCI 4和WCI 12均与IG重链上的蛋白决定簇（相对分子质量类似于70，000）反应。淋巴样细胞的免疫荧光显微镜和流式细胞术分析表明B细胞和浆细胞的三种不同亚群：WCI 4（+）12（-）细胞、WCI 4（-）12（+）细胞和WCI 4（+）12（+）细胞。用ELISPOT法在原肾和脾细胞悬液中也证实了WCI 4（-）12（+）和WCI 4（+）12（+）抗DNP抗体分泌细胞。鲤鱼血清的亲和层析和I-125标记的外周血白细胞（PBL）膜蛋白的连续免疫沉淀仅表明存在两种抗原性不同的IG分子，即，WC 14（-）12（+）和WC 14（+）12（+）分子。WCI 4（+）12（-）分子不能通过亲和层析或免疫沉淀检测。在个体发育过程中，在脾脏和前肾中发现WCI 4（+）12（-）和WCI 4（-）12（+）细胞的百分比发生变化。在这些器官中，WCI 4（+）12（-）细胞在2周龄时形成了大多数B细胞，但这种细胞类型的百分比在个体发育过程中下降。另一方面，WCI 4（-）12（+）细胞的百分比在发育过程中增加，并且这些细胞从13周开始成为B细胞的主要群体。WCI 4（+）12（+）细胞的比例在个体发育过程中几乎保持不变。血液中B细胞亚群的分布在所有年龄段或多或少是稳定的。IG异质性在鱼类，特别是鲤鱼的功能意义进行了讨论。
B cell and immunoglobulin (Ig) heterogeneity was demonstrated in carp, Cyprinus carpio L., using two monoclonal antibodies (MAbs; WC14, WCI12) produced against carp serum Ig. Immunochemical results showed that both WCI4 and WCI12 react with a protein determinant on the heavy chain of Ig (relative molecular mass similar to 70,000). Immunofluorescence microscopic and flow cytometric analyses of lymphoid cells suggest three distinct subpopulations of B cells and plasma cells: WCI4(+)12(-) cells, WCI4(-)12(+) cells, and WCI4(+)12(+) cells. WCI4(-)12(+) and WCI4(+)12(+) anti-DNP antibody-secreting cells were also demonstrated with the ELISPOT assay in pronephros and spleen cell suspensions from primary immunised carp. Affinity chromatography of carp serum and sequential immunoprecipitation of I-125-labelled peripheral blood leucocyte (PBL) membrane proteins only indicated the presence of two antigenically different Ig molecules, i.e., WCI4(-)12(+) and WCI4(+)12(+) molecules. WCI4(+)12(-) molecules could not be detected by affinity chromatography or immunoprecipitation. During ontogeny, a shift in percentages of WCI4(+)12(-) and WCI4(-)12(+) cells was found in the spleen and the pronephros. In these organs, WCI4(+)12(-) cells formed the majority of B cells at 2 weeks of age, but the percentages of this cell type decreased during ontogeny. On the other hand, the percentages of WCI4(-)12(+) cells increased during development, and these cells became the major population of B cells from 13 weeks onward. The proportion of WCI4(+)12(+) cells remained almost constant during ontogeny. The distribution of B cell subpopulations in blood was more or less stable at all ages. The functional significance of Ig heterogeneity in fish and in particular carp is discussed.