A novel marine compound xyloketal B protects against oxidized LDL-induced cell injury in vitro
A novel marine compound xyloketal B protects against oxidized LDL-induced cell injury in vitro
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一种新型海洋化合物 xyloketal B 在体外可防止氧化 LDL 诱导的细胞损伤
DOI：
10.1016/j.bcp.2009.05.029
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发表时间：
2009-10-15
影响因子：
5.8
通讯作者：
Wang, Guan-Lei
中科院分区：
文献类型：
--
作者：
Chen, Wen-Liang;Qian, Yan;Wang, Guan-Lei
木缩酮B是从红树林真菌Xylaria sp.（no.2508）中分离得到的具有独特化学结构的新型海洋化合物。用木糖缩酮B（0.63-40 μ M）预处理显著改善oxLDL（150 μ g/ml）诱导的人脐静脉内皮细胞（HUVECs）损伤，而无毒性或增殖作用。木糖缩酮B浓度依赖性地减弱oxLDL诱导的ROS生成、过氧亚硝酸盐形成和Bcl-2表达的降低。此外，木缩酮B显著抑制NADPH氧化酶活性，以及gp 91 phox和p47 phox的mRNA表达。此外，木缩酮B单独增加一氧化氮（NO）的产生。总的来说，这些数据表明，木缩酮B保护oxLDL诱导的内皮氧化损伤可能通过抑制NADPH氧化酶衍生的ROS产生，促进NO产生和恢复Bcl-2表达，使其成为一个有前途的化合物，用于进一步评估动脉粥样硬化的治疗。(C)2009 Elsevier Inc. All rights reserved.
Xyloketal B is a novel marine compound with unique chemical structure isolated from mangrove fungus Xylaria sp. (no. 2508). Pretreatment with xyloketal B (0.63-40 mu M) significantly improved oxLDL (150 mu g/ml)-induced injury in human umbilical vein endothelial cells (HUVECs) without either toxic or proliferative effects. Xyloketal B concentration-dependently attenuated oxLDL-induced ROS generation, peroxynitrite formation and decrease of Bcl-2 expression. In addition, xyloketal B significantly inhibited NADPH oxidase activity, as well as mRNA expression of gp91phox and p47phox. Furthermore, xyloketal B alone augmented the production of nitric oxide (NO). Collectively, these data indicate that xyloketal B protects against oxLDL-induced endothelial oxidative injury probably through inhibiting NADPH oxidase-derived ROS generation, promoting NO production and restoring Bcl-2 expression, making it a promising compound for further evaluation in the treatment of atherosclerosis. (C) 2009 Elsevier Inc. All rights reserved.