Mycorrhiza-induced resistance against the root-knot nematode Meloidogyne incognita involves priming of defense gene responses in tomato
Mycorrhiza-induced resistance against the root-knot nematode Meloidogyne incognita involves priming of defense gene responses in tomato
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DOI：
10.1016/j.soilbio.2013.01.013
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发表时间：
2013-05-01
影响因子：
9.7
通讯作者：
Gianinazzi-Pearson, V.
中科院分区：
文献类型：
--
作者：
Vos, C.;Schouteden, N.;Gianinazzi-Pearson, V.
丛枝菌根真菌(AMF)对引起植物根结线虫根瘤形成的根结线虫具有很大的生防潜力，但对线虫生物防治的分子机制知之甚少。本研究利用抑制消减杂交技术(SSH)对番茄根部特异上调的植物基因进行了研究。Marmande)，在土壤接种南方根结线虫幼虫12天后，由AMF Glomus Mosseae(BEG 12)预先定殖。与非菌根相比，菌根中的线虫侵染率显著降低，所鉴定的基因主要在防御、信号转导和蛋白质合成与修饰方面。与只有菌根或只有线虫侵染的植物相比，在生防互作中一些特定的与防御相关的植物基因的表达水平更高，这为菌根诱导植物防御反应的存在提供了依据。综上所述，通过对菌根番茄与南方根结线虫生防互作中基因表达上调的研究，对菌根诱导番茄对根结线虫病抗性的分子机制有了新的认识。尤其是苯丙烷途径和活性氧(ROS)代谢的参与可以解释番茄根部根结线虫侵染减少的原因，这一过程也被报道在植物对线虫的抗性中发挥关键作用。(C)2013爱思唯尔有限公司。保留所有权利。
Arbuscular mycorrhizal fungi (AMF) have great potential as biocontrol organisms against the root-knot nematode Meloidogyne incognita which causes severe gall formation in plants, but knowledge about the underlying molecular mechanisms involved in the biocontrol of nematodes is scarce. In the present study, suppression subtractive hybridization (SSH) was used to investigate plant genes that are specifically up-regulated in tomato roots (Solanum lycopersicum cv. Marmande) pre-colonized by the AMF Glomus mosseae (BEG 12) and 12 days after soil inoculation with M. incognita juveniles. Nematode infection was significantly lower in the mycorrhizal roots as compared to the non-mycorrhizal roots, and identified genes were classified mainly in the categories of defense, signal transduction and protein synthesis and modification. The higher expression of a selection of defense-related plant genes specifically in the biocontrol interaction compared to in plants that were only mycorrhizal or only nematode-infected was confirmed, which pleads for the existence of mycorrhiza-induced priming of plant defense responses. In conclusion, by focusing on up-regulated gene expression in the biocontrol interaction between mycorrhizal tomato and M. incognita, new insights were found into the molecular mechanisms underlying the mycorrhiza-induced resistance against root-knot nematodes. In particular, the involvement of the phenylpropanoid pathway and reactive oxygen species (ROS) metabolism could explain the reduced root-knot nematode infection in mycorrhizal tomato roots, processes that have also been reported to play a pivotal role in plant resistance to nematodes. (C) 2013 Elsevier Ltd. All rights reserved.