Glypican-3 antibody functionalized Prussian blue nanoparticles for targeted MR imaging and photothermal therapy of hepatocellular carcinoma
Glypican-3 antibody functionalized Prussian blue nanoparticles for targeted MR imaging and photothermal therapy of hepatocellular carcinoma
复制标题
Glypican-3 抗体功能化普鲁士蓝纳米粒子用于肝细胞癌的靶向 MR 成像和光热治疗
DOI：
10.1039/c4tb00516c
复制
发表时间：
2014-01-01
影响因子：
7
通讯作者：
Liu, Jingfeng
中科院分区：
文献类型：
--
作者：
Li, Zhenglin;Zeng, Yongyi;Liu, Jingfeng
核磁共振引导光热疗法正成为一种被广泛接受的微创技术。在这项研究中，glypican-3单克隆抗体功能化普鲁士蓝纳米颗粒（抗gpc3 - pbnps）被开发为一种新的治疗药物，用于肝细胞癌的靶向磁共振成像和光热治疗。通过SEM、TEM和Vis-NIR吸收光谱对抗gpc3 - pbnps的物理性质进行了表征，结果表明所制备的纳米探针形成了均匀的纳米立方体，平均直径为21 nm。共聚焦荧光显微镜和ICP-MS证实了HepG2细胞通过受体介导的内吞作用显著提高靶向细胞摄取效率。此外，所制备的纳米探针具有较高的光热细胞毒性、优异的MR成像对比度增强能力和生物相容性。因此，所制备的抗gpc3 - pbnps可作为一种有前景的纳米探针，用于肝癌的靶向MRI诊断和高效光热治疗。
MRI-guided photothermal therapy is becoming a more widely accepted minimally invasive technique. In this study, glypican-3 monoclonal antibody functionalized Prussian blue nanoparticles (antiGPC3-PBNPs) were developed as a novel theranostic agent for the targeted MR imaging and photothermal therapy of hepatocellular carcinoma. The physical properties of the antiGPC3-PBNPs were characterized by SEM, TEM and Vis-NIR absorption spectra, which showed that the developed nanoprobe formed well defined nanocubes with an average diameter of 21 nm. The significantly increased targeting cellular uptake efficiency in HepG2 cells via receptor-mediated endocytosis was confirmed by confocal fluorescence microscopy and ICP-MS. Furthermore, the high photothermal cytotoxicity, excellent MR imaging contrast enhancement ability and biocompatibility of the developed nanoprobe were also confirmed. Hence, the developed antiGPC3-PBNPs could be used as a promising nanoprobe for targeted MRI diagnosis and efficient photothermal therapy for hepatocellular carcinoma.