Expression of natriuretic peptides in rat Müller cells
Expression of natriuretic peptides in rat Müller cells
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DOI：
10.1016/j.neulet.2004.04.090
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发表时间：
2004-07
影响因子：
2.5
通讯作者：
L. Cao;Yong-Chun Yu;Jing-Wei Zhao;Xiong-Li Yang
中科院分区：
文献类型：
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作者：
L. Cao;Yong-Chun Yu;Jing-Wei Zhao;Xiong-Li Yang
研究表明，利钠肽(NPs)可以调节神经元的活动。应用免疫组织化学和激光共聚焦显微镜技术，观察了心钠素(ANP)、脑钠素(BNP)和C型神经肽(CNP)在大鼠视网膜中的表达。我们的结果表明，这些多肽在神经视网膜中的表达存在差异。ANP、BNP和CNP免疫反应(IR)在神经节细胞的外层和内层及内核层的大量神经元上有明显的表达，而在一些神经节细胞中仅有BNP和CNP表达，而ANP-IR则不表达。此外，NPs和Vimentin的双重标记显示，ANP、BNP和CNP在Müller细胞中以不同的轮廓表达。BNP的标记在主干、主要过程中相当强烈，但在末端几乎检测不到。ANP的表达谱相似，但水平要低得多。相反，内侧视网膜的终足和主要突起呈CNP强阳性，而外侧视网膜的主干和其他主要突起的标记要少得多。这些结果提出了一种可能性，当NPs从Müler细胞中释放出来时，可能执行层依赖的功能。
Natriuretic peptides (NPs) have been shown to modulate neuronal activities. By immunohistochemistry and confocal microscopy, we examined expression of atrial NP (ANP), brain NP (BNP) and C-type NP (CNP) in rat retina. Our results showed that these peptides were differentially expressed in the neural retina. While strong ANP-, BNP- and CNP-immunoreactivity (IR) was clearly seen in the outer and inner plexiform layers and on numerous neurons in the inner nuclear layer, BNP- and CNP-, but not ANP-IR, was present in some ganglion cells. Furthermore, ANP, BNP and CNP were expressed in Müller cells with distinct profiles, as shown by double labeling of NPs and vimentin. Labeling for BNP was rather strong in the main trunks, major processes, but hardly detectable in the endfeet. The expression profile for ANP was similar, but with a much lower level. On the contrary, the endfeet and major processes in the inner retina were strongly CNP-positive, with the main trunks and other major processes in the outer retina much less labeled. These results raise a possibility that NPs, when released from Müller cells, may perform layer dependent functions.