Biochemical gas sensor (bio-sniffer) for ultrahigh-sensitive gaseous formaldehyde monitoring.
Biochemical gas sensor (bio-sniffer) for ultrahigh-sensitive gaseous formaldehyde monitoring.
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DOI：
10.1016/j.bios.2010.07.099
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发表时间：
2010-10
影响因子：
12.6
通讯作者：
H. Kudo;Yuki Suzuki;T. Gessei;D. Takahashi;T. Arakawa;K. Mitsubayashi
中科院分区：
文献类型：
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作者：
H. Kudo;Yuki Suzuki;T. Gessei;D. Takahashi;T. Arakawa;K. Mitsubayashi
研制了一种超高灵敏度的光纤生化气体传感器（生物嗅探器），用于室内甲醛的连续监测。生物嗅探器测量气态甲醛作为烟酰胺腺嘌呤二核苷酸（NADH）的荧光，烟酰胺腺嘌呤二核苷酸是甲醛脱氢酶（NADH）反应的产物。生物嗅探器装置是通过将具有NADH固定膜的流动池连接到光纤NADH测量系统上来构建的。NADH测量系统利用峰值发射为335 nm的紫外发光二极管（UV-LED）作为激发光源。将激发光引入光纤探针，用光电倍增管同心地测量通过将甲醛蒸气施加到H2 O2 DH膜产生的邻近NADH的荧光发射。使用标准气体发生器对生物嗅探器进行评估。研究了对其他化学物质的响应、校准范围和选择性。含有NAD+的循环磷酸盐缓冲液可用于甲醛蒸气的连续监测。生物嗅探器的校准范围为2.5ppb ~ 10 ppm，涵盖了世界卫生组织的指导值（80 ppb）。还证实了由于BADDH的比活性而对其他气态物质的高选择性。考虑到其高灵敏度，生物嗅探器的一个可能的应用是连续的室内甲醛监测，以提供健康的住宅气氛。
An ultrahigh-sensitive fiber-optic biochemical gas sensor (bio-sniffer) for continuous monitoring of indoor formaldehyde was constructed and tested. The bio-sniffer measures gaseous formaldehyde as fluorescence of nicotinamide adenine dinucleotide (NADH), which is the product of formaldehyde dehydrogenase (FALDH) reaction. The bio-sniffer device was constructed by attaching a flow cell with a FALDH immobilized membrane onto a fiber-optic NADH measurement system. The NADH measurement system utilizes an ultraviolet-light emitting diode (UV-LED) with peak emission of 335nm as an excitation light source. The excitation light was introduced to an optical fiber probe, and fluorescence emission of neighboring NADH, which was produced by applying formaldehyde vapor to the FALDH membrane, was concentrically measured with a photomultiplier tube. Assessment of the bio-sniffer was carried out using a standard gas generator. Response, calibration range and selectivity to other chemical substances were investigated. Circulating phosphate buffer, which contained NAD+, available for continuous monitoring of formaldehyde vapor. The calibration range of the bio-sniffer was 2.5ppb to 10ppm, which covers the guideline value of the World Health Organization (80ppb). High selectivity to other gaseous substances due to specific activity of FALDH was also confirmed. Considering its high sensitivity, a possible application of the bio-sniffer is continuous indoor formaldehyde monitoring to provide healthy residential atmosphere.