Kobuviruses carried by Rattus norvegicus in Guangdong, China
Kobuviruses carried by Rattus norvegicus in Guangdong, China
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中国广东省褐家鼠携带科布病毒
DOI：
10.1186/s12866-020-01767-x
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发表时间：
2020
期刊：
影响因子：
4.2
通讯作者：
Qing Chen
中科院分区：
文献类型：
--
作者：
Fang;Minyi Zhang;Huan He;Wen;Yong;Qing Chen
鼠科布病毒（MuKV）是2011年在美国首次在鼠啮齿动物中发现的新发现的小核糖核酸病毒。关于鼠啮齿类动物中MuKV流行病学的信息很少。因此，我们对中国广东地区大鼠Kobuvirus的流行情况和基因组特征进行了调查。从广东省223份褐家鼠粪便标本中检出28份（12.6%）Kobuvirus。基于部分3D和完整VP 1序列的系统发育分析表明，本研究中获得的大鼠kobuvirus与其他地理区域报道的大鼠/小鼠kobuvirus在遗传上密切相关。获得了两个接近全长的大鼠kobuvirus基因组（MM 33，GZ 85），并对它们进行了系统发育分析，发现它们彼此具有非常高的核苷酸/氨基酸同源性（95.4%/99.4%）和来自污水的序列（分别为86.9%/93.5%和87.5%/93.7%）。与原始的艾奇病毒A株，如人kobuvirus比较，显示氨基酸同一性值约为80%。我们的研究结果表明，大鼠kobuvirus具有独特的遗传特征，从其他艾奇病毒A病毒。此外，鼠科布病毒可以通过污水传播。
Murine kobuviruses (MuKV) are newly recognized picornaviruses first detected in murine rodents in the USA in 2011. Little information on MuKV epidemiology in murine rodents is available. Therefore, we conducted a survey of the prevalence and genomic characteristics of rat kobuvirus in Guangdong, China. Fecal samples from 223 rats (Rattus norvegicus) were collected from Guangdong and kobuviruses were detected in 12.6% (28) of samples. Phylogenetic analysis based on partial 3D and complete VP1 sequence regions showed that rat kobuvirus obtained in this study were genetically closely related to those of rat/mouse kobuvirus reported in other geographical areas. Two near full-length rat kobuvirus genomes (MM33, GZ85) were acquired and phylogenetic analysis of these revealed that they shared very high nucleotide/amino acids identity with one another (95.4%/99.4%) and a sewage-derived sequence (86.9%/93.5% and 87.5%/93.7%, respectively). Comparison with original Aichivirus A strains, such human kobuvirus, revealed amino acid identity values of approximately 80%. Our findings indicate that rat kobuvirus have distinctive genetic characteristics from other Aichivirus A viruses. Additionally, rat kobuvirus may spread via sewage.