Rapid and quantitative detection of CpG-methylation status using TaqMan PCR combined with methyl-binding-domain polypeptide
Rapid and quantitative detection of CpG-methylation status using TaqMan PCR combined with methyl-binding-domain polypeptide
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DOI：
10.1016/j.clinbiochem.2009.03.017
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发表时间：
2009-07-01
影响因子：
2.8
通讯作者：
Oka, Masaaki
中科院分区：
文献类型：
--
作者：
Kimura, Naoki;Moribe, Toyoki;Oka, Masaaki
目的：为了评估CpG甲基化作为癌症诊断分子标记的医学适用性，我们建立了一种基于TaqMan PCR结合甲基结合结构域多肽2的DNA甲基化检测新体系。设计和方法：我们通过检测两种肿瘤抑制基因RASSFIA和APC在10对肝细胞癌（HCC）和相应的非肿瘤肝组织中的甲基化状态来评估该方法的诊断适用性。结果：TaqMan PCR检测的20份临床样本的甲基化水平呈显著正相关(R=0.814， P
Objectives: To assess the medical applicability of CpG methylation as molecular markers for cancer diagnosis, we established a new system to determine DNA methylation based on TaqMan PCR combined with a methyl-binding-domain polypeptide 2.Design and methods: We evaluated the diagnostic applicability of this approach by examining the methylation status of two tumor suppressor genes, RASSFIA and APC, in 10 paired hepatocellular carcinoma (HCC) and the corresponding non-tumor liver tissues.Results: Methylation levels of total 20 clinical samples measured by the TaqMan PCR assay showed a significantly positive correlation (R=0.814, P