Skeletal Muscle Denervation: Sciatic and Tibial Nerve Transection Technique
Skeletal Muscle Denervation: Sciatic and Tibial Nerve Transection Technique
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骨骼肌去神经支配：坐骨神经和胫神经横断技术
DOI：
10.1007/978-1-0716-3036-5_16
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发表时间：
2023
影响因子：
--
通讯作者：
Ohashi Kazuya
中科院分区：
文献类型：
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作者：
Goto Katsumasa;Ohashi Kazuya
神经切断模型是通过在啮齿动物中使骨骼肌失神经支配而制备的骨骼肌萎缩的已建立和验证的实验模型。虽然许多去神经技术可用于大鼠，但各种转基因和基因敲除小鼠的发展也导致了神经横断小鼠模型的广泛使用。骨骼肌去神经实验扩展了我们对神经活动和/或神经营养因子在骨骼肌可塑性中的生理作用的认识。坐骨神经或胫神经的去神经是小鼠和大鼠中常见的实验程序，因为这些神经可以毫不费力地切除。最近发表了越来越多的关于在小鼠中使用胫神经横断技术的实验的报告。在这一章中，我们演示和解释的程序用于横断小鼠坐骨神经和胫神经。
The nerve transection model is an established and validated experimental model of skeletal muscle atrophy prepared by denervating the skeletal muscle in rodents. While a number of denervation techniques are available in rats, the development of various transgenic and knockout mice has also led to the wide use of mouse models of nerve transection. Skeletal muscle denervation experiments expand our knowledge of the physiological role of nerval activity and/or neurotrophic factors in the plasticity of skeletal muscle. The denervation of the sciatic or tibial nerve is a common experimental procedure in mice and rats, as these nerves can be resected without great difficulty. An increasing number of reports have recently been published on experiments using a tibial nerve transection technique in mice. In this chapter, we demonstrate and explain the procedures used to transect the sciatic and tibial nerves in mice.