Does prostacyclin (PGI2) regulate human arterial intima smooth muscle cell proliferation in early atherogenesis?
Does prostacyclin (PGI2) regulate human arterial intima smooth muscle cell proliferation in early atherogenesis?
复制标题
前列环素 (PGI2) 是否调节早期动脉粥样硬化形成中的人动脉内膜平滑肌细胞增殖？
DOI：
10.1159/000158235
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
W. Auerswald
中科院分区：
文献类型：
--
作者：
H. Sinzinger;K. Silberbauer;W. Auerswald
PGI2 synthesis by cultured aortic smooth muscle cells (SMC) has been recently reported (3, 12). After the discovery of PG12 formation by vascular endothelial cells (13), it was later reported that the subendothelial SMC in rabbit (14), rat (11) and human (16) is also able to generate important amounts of PGI2. In vitro experiments demonstrated that different dos ages of PG12 either contract (7) or dilate SMC strips (4). Therefore, it has been concluded that PG12 in physiological concentrations regulates the vascular tone in vivo (1, 15). However, the estimation of PGI2 synthesis by different types of SMC in the vascular wall (2, 5) has not yet been carried out.Our investigations were carried out using four splenic arteries from patients (aged 37—52 years) removed during surgery. The vessel wall layers were microdissected in ice-cold Tris-HCl buffer (0.5 mol/T, pH 7.1) immediately after removing (within 30 min). Intimal tissue sam ples each 8—16 mg (wet weight) were bioas sayed for the PGI2 production as described before (16). The activity was estimated by
