Activin Inhibits Cell Growth and Induces Differentiation in Human Retinoblastoma Y79 Cells
Activin Inhibits Cell Growth and Induces Differentiation in Human Retinoblastoma Y79 Cells
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DOI：
10.1080/02713680903007147
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发表时间：
2009-01-01
影响因子：
2
通讯作者：
Yamamoto, Teiko
中科院分区：
文献类型：
--
作者：
Kanno, Chikako;Horie, Kuniko;Yamamoto, Teiko
目的：激活素是转化生长因子-β超家族的一员，在细胞分化和细胞凋亡中发挥一定的作用。我们研究了激活素对视网膜母细胞瘤细胞系的影响。材料和方法：采用视网膜母细胞瘤细胞系Y79。用RT-PCR、免疫荧光和荧光素酶报告实验研究激活素在细胞内的信号转导。荧光细胞存活率法检测激活素对细胞生长的影响。为确定激活素对细胞凋亡的影响，我们对裂解的PARP进行了原位末端标记法和免疫荧光研究。RT-PCR和Western blotting检测激活素对细胞分化的影响。结果：激活素在Y79细胞中存在细胞内信号转导。激活素对Y79细胞生长有抑制作用。激活素诱导视网膜亮氨酸拉链(NRL)在mRNA和蛋白水平表达。结论：NRL是视杆细胞感光细胞发育过程中的特异性基因，也是分化为视杆细胞所必需的基因，提示激活素影响视网膜母细胞瘤细胞向视杆细胞的分化。
Purpose: Activin is a member of the transforming growth factor-beta (TGF-beta) superfamily and exerts certain effects on differentiation and apoptosis. We investigated the effects of activin on retinoblastoma cell line. Materials and Methods: We used retinoblastoma cell line Y79. Intracellular signal transduction of activin was investigated with RT-PCR, immunofluorescence study, and luciferase reporter assay. The effect of activin on cell growth was examined with fluorescence cell viability assays. To determine the effect of activin on apoptosis, a TUNEL assay and an immunofluorescence study of cleaved PARP were performed. The effect of activin on cell differentiation was examined with RT-PCR and Western blotting. Results: Intracellular signal transduction of activin was confirmed in Y79 cells. Activin inhibited Y79 cell growth. Activin induced the expression of neural retina leucine zipper (Nrl) at the mRNA and protein levels. Conclusions: Nrl is a specific gene in rod photoreceptor development and is a gene indispensable to differentiation into rod photoreceptors, so the present results suggest that activin affects the differentiation of retinoblastoma cells into rod photoreceptor cells.