Optical recording of light-evoked calcium signals in the functionally intact retina
Optical recording of light-evoked calcium signals in the functionally intact retina
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DOI：
10.1073/pnas.96.12.7035
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发表时间：
1999-06-08
影响因子：
11.1
通讯作者：
Detwiler, PB
中科院分区：
文献类型：
--
作者：
Denk, W;Detwiler, PB
利用荧光指示剂的双光子激发，我们通过最大限度地激活或漂白光感受器，在不破坏视网膜光敏性的情况下，测量了视网膜神经节和无长突细胞中的钙浓度瞬变。这使得立即评估细胞形态和研究视觉刺激引起的钙信号成为可能。在930~990 nm的激发波长范围内，可长时间观察单个树突状突起的钙动力学，没有恶化，且几乎没有明显的光毒性。光诱发的钙增加在神经节和无长突细胞神经突中被分解，使得利用光学记录来研究钙信号与视网膜功能之间的关系成为可能。
Using two-photon excitation of Fluorescent indicator dyes, we measured calcium concentration transients in retinal ganglion and amacrine cells without destroying the light sensitivity of the retina by maximally activating or bleaching the photoreceptors. This allowed an immediate assessment of the cellular morphology and study of the calcium signals evoked by visual stimuli. Calcium dynamics in individual dendritic processes could be examined for extensive periods without deterioration and with little apparent phototoxicity at excitation wavelengths of from 930 to 990 nm. Light-evoked increases in calcium were resolved in ganglion- and amacrine-cell neurites, making it possible to use optical recording to study the relationship between calcium signaling and retinal function.